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Identification of mycobiota from Tunisian olives and determination of their mycotoxigenic profile

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dc.contributor.authorHamdi, Mohamed
dc.contributor.authorBejaoui, Hend
dc.contributor.authorRodrigues, Paula
dc.date.accessioned2018-02-14T12:01:48Z
dc.date.available2018-02-14T12:01:48Z
dc.date.issued2017
dc.description.abstractIn the Mediterranean basin, a large number of olive varieties are produced and cultivated. In Tunisia, olive culture represents one of the most significant agricultural products that is produced annually. Storage under inadequate conditions poses serious problems concerning fungal contamination, with consequent defects and potential mycotoxin production in olives and olive oils. In this study, we aimed to identify and characterize a selected group of fungi previously isolated from olives from Tunisian groves. For this purpose, 28 fungi were selected for identification by a polyphasic approach consisting of morphological identification on Malt Extract Agar (MEA) and Czapek Yeast Autolysate (CYA); physiological identification by the Erlich test (for the genus Penicillium only); and molecular identification by sequencing of the ITS region of the rRNA gene. Mycotoxigenic ability of fungi was screened on Coconut Agar Medium (CAM). Methanolic extracts of those fungi showing fluorescence on CAM wereobtained from CYA cultures and analysed by HPLC. From morphological and molecular identification, eight species belonging to the genera Alternaria, Aspergillus, Fusarium, Penicillium, Pleospora and Tricothecium and were identified. Results showed several isolates were able to produce toxins on CAM, by showing green or orange fluorescence. HPLC analysis are being developed.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationHamdi, Mohamed; Bejaoui, Hend; Rodrigues, Paula (2017). Identification of mycobiota from Tunisian olives and determination of their mycotoxigenic profile. In Journées Scientifiques Internationales sur la Valorisation des Bioressources. Monastir, Tunisiapt_PT
dc.identifier.urihttp://hdl.handle.net/10198/15728
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMycotoxinspt_PT
dc.subjectPenicilliumpt_PT
dc.subjectFood safetypt_PT
dc.subjectOlivespt_PT
dc.titleIdentification of mycobiota from Tunisian olives and determination of their mycotoxigenic profilept_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceMonastir, Tunisiapt_PT
oaire.citation.titleJournées Scientifiques Internationales sur la Valorisation des Bioressourcespt_PT
person.familyNameRodrigues
person.givenNamePaula
person.identifier.ciencia-id2312-18AE-DA42
person.identifier.orcid0000-0002-3789-2730
person.identifier.scopus-author-id7102639646
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicationb864b8c1-d66a-419c-b1ac-e6193f93eca6
relation.isAuthorOfPublication.latestForDiscoveryb864b8c1-d66a-419c-b1ac-e6193f93eca6

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