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Orientador(es)
Resumo(s)
The present work focused on the development of a process of sorption-desorption to
purify (E)-resveratrol found in winemaking residues, specifically from the crude extract of
grape stems. The core element of this process is a photo-molecularly imprinted sorbent
(MIS) synthesized by means of the molecular imprinting technique (MIT). This sorbent is a
4-vinylpyridine rich polymer network which binds (E)-resveratrol at stereospecific sites.
Comparison of sorption isotherms of the MIS and its counterpart non-imprinted sorbent
(NIS) shows the higher capability of the MIS to bind (E)-resveratrol. It is noteworthy that
the size of MIS particles was larger than to those of the NIS (as found by scanning electron
microscopy analysis) facilitating its use in sorption-desorption processes without
reaching a high backpressure. The MIS was then used to purify (E)-resveratrol from grape
stems extract in a two-step purification procedure through a simple device. The initial
purification step permitted to increase the purity of (E)-resveratrol from 29% to 78% (34%
of recovery), whereas a further one took it to 87% (70% of recovery). The purified fractions
were obtained in hydroalcoholic medium and contained other bioactive stilbenes such as
(E)-Ɛ-viniferin. The results of this research provide a compelling proof of concept for
room-temperature photopolymerization, enabling the development of highly efficient
imprinted polymers as sorbents for purifying bioactive compounds from agricultural residues.
This significant advancement paves the way for its large-scale implementation in
a circular bioeconomy approach.
Descrição
Palavras-chave
Photo-molecularly imprinted sorbent (E)-resveratrol purification Sorption-desorption processes Winemaking residues Circular bioeconomy
Contexto Educativo
Citação
Bzainia, Amir; Dias, Rolando; Costa, Mário Rui (2023). A simple process to purify (E)-resveratrol from grape stems with a photo-molecularly imprinted sorbent. Food and Bioproducts Processing. ISSN 0960-3085. 142, p. 1-16
Editora
Elsevier