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Chemical composition, antioxidant activity, and diuretic effect of Moroccan fresh bee pollen in rats

2020Journal article Open access
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dc.contributor.authorEl Ghouizi, Asmae
dc.contributor.authorEl Menyiy, Nawal
dc.contributor.authorFalcão, Soraia
dc.contributor.authorVilas-Boas, Miguel
dc.contributor.authorLyoussi, Badiâa
dc.date.accessioned2018-02-19T10:00:00Z
dc.date.accessioned2021-02-27T17:21:00Z
dc.date.available2018-01-19T10:00:00Z
dc.date.available2021-02-27T17:21:00Z
dc.date.issued2020
dc.description.abstractThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. Aim: This study investigated the chemical composition, antioxidant activity, and diuretic effect of Moroccan aqueous extract of fresh bee pollen (AEFBP) in normal rats. Materials and Methods: The chemical composition of the extracted bioactive compounds was assessed using liquid chromatography with diode array detection coupled to electrospray ionization (ESI) tandem mass spectrometry (LC/DAD/ ESI-MSn). 2,2-diphenyl-1-picrylhydrazyl and the reducing power were used to assess the antioxidant properties of the extract, together with the determination of total phenols and flavonoids. To assess the diuretic effect, 20 normal rats were divided into five groups: The first was a control group administered by distilled water (10 mL/kg body weight), the second group received furosemide (10 mg/kg body weight), the third group received 100 mg/kg body weight of AEFBP, the fourth group received 250 mg/kg body weight of AEFBP, and the fifth group received 500 mg/kg body weight of AEFBP for 30 days. Toward the end of this experiment, urine output was measured, and plasma and urine were sampled to analyze creatinine, potassium, chloride, and sodium levels. Results: N1,N5,N10-tri-p-coumaroylspermidine is a spermidine derivative and was the main compound in this sample, in a total of 19 compounds identified, including flavonoids, glucoside flavonoids, and methylated derivatives. Force feeding with the AEFBP induced a significant increase in urine output and urinary electrolyte levels with a dependent dose-effect without changes in plasma electrolytes, whereas furosemide decreased plasma potassium. Conclusion: Moroccan fresh bee pollen extract contains flavonols and spermidines that induce a potential antioxidant activity related to significant diuretic effect without changes in plasma composition.en_EN
dc.description.sponsorshipThe authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support by national funds FCT/MCTES to CIMO (UIDB/00690/2020). National funding by FCTFoundation for Science and Technology, through the institutional scientific employment program-contract with Soraia I. Falcão and to the Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health and Quality of Life (SNAMOPEQ), Faculty of Sciences Dhar El Mehraz, University Sidi Mohamed Ben Abdallah, Fez, Morocco for providing the facilities to carry out the present research work.
dc.description.versioninfo:eu-repo/semantics/publishedVersionen_EN
dc.identifier.citationEl Ghouizi, Asmae; El Menyiy, Nawal; Falcão, Soraia I.; Vilas-Boas, Miguel; Lyoussi, Badiaa (2020). Chemical composition, antioxidant activity, and diuretic effect of Moroccan fresh bee pollen in rats. Veterinary World. ISSN 0972-8988. 13:7, p. 1251-1261en_EN
dc.identifier.doi10.14202/vetworld.2020.1251-1261en_EN
dc.identifier.issn0972-8988
dc.identifier.urihttp://hdl.handle.net/10198/23409
dc.language.isoeng
dc.peerreviewedyesen_EN
dc.subjectAntioxidant activityen_EN
dc.subjectDiuresisen_EN
dc.subjectFresh bee pollenen_EN
dc.subjectLiquid chromatography-mass spectrometryen_EN
dc.subjectPhenolamidesen_EN
dc.titleChemical composition, antioxidant activity, and diuretic effect of Moroccan fresh bee pollen in ratsen_EN
dc.typejournal article
dspace.entity.typePublication
person.familyNameFalcão
person.familyNameVilas-Boas
person.givenNameSoraia
person.givenNameMiguel
person.identifier711432
person.identifier1693134
person.identifier.ciencia-idD41A-6BED-4CBB
person.identifier.ciencia-idA918-C6FF-81A4
person.identifier.orcid0000-0003-3735-6951
person.identifier.orcid0000-0002-8665-5280
person.identifier.ridI-5949-2013
person.identifier.scopus-author-id14832459200
person.identifier.scopus-author-id6602648497
rcaap.rightsopenAccessen_EN
rcaap.typearticleen_EN
relation.isAuthorOfPublication5746ab3e-8ffe-4e26-9a41-8392cf031f28
relation.isAuthorOfPublicationf58280c8-298b-4e4c-9f7c-4517300d3382
relation.isAuthorOfPublication.latestForDiscovery5746ab3e-8ffe-4e26-9a41-8392cf031f28

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