Publication
Development and evaluation of a normal phase liquid chromatographic method for the determination of tocopherols and tocotrienols in walnuts
| dc.contributor.author | Amaral, Joana S. | |
| dc.contributor.author | Casal, Susana | |
| dc.contributor.author | Seabra, Rosa M. | |
| dc.contributor.author | Oliveira, Beatriz | |
| dc.date.accessioned | 2008-05-28T10:35:48Z | |
| dc.date.available | 2008-05-28T10:35:48Z | |
| dc.date.issued | 2005 | |
| dc.description.abstract | A high performance liquid chromatographic (HPLC) method for the determination of tocopherols and tocotrienols in walnut samples is described. The compounds were extracted with n-hexane, using a simple solid-liquid extraction procedure. Tocol was used as internal standard and BHT as anti-oxidant. The ehromatographic separation was achieved using an Inertsil 5 SI normal phase column operating with isocratic elution of n-hexane/1,4-dioxane (96.5:3.5, v/v), at a flow rate of 0.7 mL/min. The effluent was monitored by a series arrangement of a diodearray detector followed by a fluorescence detector. The detection limits were low, between 0.037 and 0.266 (jig/mL. The method was precise (% CV less than2.8%), accurate (% CV less than 5.6%), and, as a general rule, the recovery values were high (mean values ranging from 93.4% to 104%). | en |
| dc.identifier.citation | Amaral, J.S.; Casal, S.; Seabra, R. M.; Oliveira, M.B.P.P. (2005). Development and evaluation of a normal phase liquid chromatographic method for the determination of tocopherols and tocotrienols in walnuts. Journal of Liquid Chromatography and Related Technologies. ISSN 1082-6076. 28:5, p.785-795 | en |
| dc.identifier.doi | 10.1081/JLC-200048920 | |
| dc.identifier.issn | 1082-6076 | en |
| dc.identifier.slug | Journal of Liquid Chromatography and Related Technologies | en |
| dc.identifier.uri | http://hdl.handle.net/10198/632 | |
| dc.language.iso | eng | en |
| dc.language.rfc3066 | eng | en |
| dc.number | 5 | en |
| dc.pagination | 785-795 | en |
| dc.peerreviewed | yes | en |
| dc.publisher | Taylor & Francis | en |
| dc.subject | Tocopherol | en |
| dc.subject | Tocotrienol | en |
| dc.subject | Normal phase liquid chromatography | en |
| dc.subject | Walnuts | en |
| dc.title | Development and evaluation of a normal phase liquid chromatographic method for the determination of tocopherols and tocotrienols in walnuts | en |
| dc.type | journal article | |
| dc.volume | 28 | en |
| dspace.entity.type | Publication | |
| person.familyName | Amaral | |
| person.givenName | Joana S. | |
| person.identifier.ciencia-id | 5319-7DE8-BEDA | |
| person.identifier.orcid | 0000-0002-3648-7303 | |
| rcaap.rights | openAccess | en |
| rcaap.type | article | |
| relation.isAuthorOfPublication | 42be2cf4-adc4-4e7f-ac60-7aab515b38cd | |
| relation.isAuthorOfPublication.latestForDiscovery | 42be2cf4-adc4-4e7f-ac60-7aab515b38cd |