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Advisor(s)
Abstract(s)
The effect of farnesol, a sesquiterpene alcohol, on the production of laccases by Trametes
versicolor and Pycnoporus sanguineus in pineapple waste solid-state fermentation was evaluated.
Extracellular laccase production reached a maximum of 77.88 5.62 U/g (236% above control) in
farnesol-induced cultures of T. versicolor on the 17th day, whereas in a similar P. sanguineus culture,
a maximal laccase activity of 130.95 2.20 U/g (159% increase) was obtained on the 17th day. A
single 45KDa laccase was produced by both fungi under the influence of farnesol. These and other
data allow us to conclude that farnesol acted as an inducer of the same form of laccase in both fungi.
Farnesol disfavored fungal growth by increasing the lag phase, but it also clearly improved the
oxidative state of the cultures. Contrary to the results obtained previously in submerged cultures,
farnesol did not promote hyperbranching in the fungal mycelia. This is the first demonstration that
farnesol is an excellent inducer of laccases in T. versicolor and P. sanguineus in solid-state cultivation. In
quantitative terms, the results can be regarded as an excellent starting point for developing industrial
or at least pre-industrial procedures to produce laccases using T. versicolor and P sanguineus under the
stimulus of farnesol.
Description
Keywords
Lag phase Fungal biomass White-rot fungi Growth inhibition
Pedagogical Context
Citation
Backes, Emanueli; Kato-Schwartz, Camila Gabriel; Oliveira Junior, Verci A. de; Uber, Thaís Marques; Santos, Luís F. O. dos; Corrêa, Rúbia C.G.; Bracht, Adelar; Peralta, Rosane Marina (2023). Overproduction of laccase by trametes versicolor and Pycnoporus sanguineus in farnesol-pineapple waste solid fermentation. Fermentation. eISSN 2311-5637. 9:2, p. 1-12
Publisher
MDPI