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Isolation and phylogenetic analysis of two actin genes from Phytophthora cinnamomi

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dc.contributor.authorJorge, Lurdes
dc.contributor.authorDias, Teresa
dc.contributor.authorAndrade, Maria
dc.contributor.authorVaz, Madalena
dc.contributor.authorDominguez, Ángel
dc.contributor.authorChoupina, Altino
dc.date.accessioned2011-02-14T14:17:05Z
dc.date.available2011-02-14T14:17:05Z
dc.date.issued2010
dc.description.abstractActins, as the essential component of cellular microfilament, are ubiquitous and highly conserved proteins that play key roles in several basic functions of organism such as cytoskeleton morphology, cell division, cell motility, cellular signal transduction, cellular interaction and organelle movements, as well as locomotion, phagocytosis, endocytosis and exocytosis . Actins are highly conserved structural proteins, found in all eukaryotes. So, actin gene sequences are used as tools in scientific research, for example, for phylogenetic analysis. Actin in Phytophthora infestans is encoded by at least two genes, in contrast to unicellular and filamentous fungi (Candida albicans, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Kluyveromyces lactis and Filobasidiella neoformans) where there is a single gene. These genes (designated actA and actB) have been isolated from a genomic library of P. infestans. Phytophthora cinnamomi is a host-nonspecific, soilborne, pathogen of many plant species. In Portugal it is most important as a pathogen of chestnut trees. The purpose of this study was to clone and determine the phylogenetic retionships evidencided by Phytophthora cinnamomi actins. In order to isolate the actin genes, P. cinnamomi was grown in cellophane-PDA medium and genomic DNA was used as a template in PCR amplification reactions combining degenerate primers Act1, Act2, Act3 and Act4. PCR fragments were purified, cloned into pGEM-T vector and transformants were selected. Complete open reading frames (ORFs) of act1 and act2 genes were achieved by HE-TAIL PCR, and submitted to EMBL databases (Accession numbers AM412175.1 and AM412176.1). Act1 has an 1128bp ORF, encoding a deduced protein of 375aa and 41,972kDa. Act2 ORF has 1083bp and encodes a deduced protein of 360aa and 40,237kDa. Deduced amino acid sequences were analyzed using FASTA programs from EMBL databases. Act1 showed a 98.9% identity with P. melonis actB, 94.4% with P. megasperma actin and 96.0% with P. infestans actin2. Act2 showed a 98.9% identity with Pythium splendens actin and 98.6% with P. brassicae actinA.por
dc.description.sponsorshipCOMBATINTA/SP2.P11/02 Interreg IIIA – Cross-Border Cooperation Spain-Portugal; Identification, characterization and role of molecular factors associated with the mechanisms of infection of Fagaceae species by Phytophthora cinnamomi” (PTDC/AGRAAM/67628/2006) FCT
dc.identifier.citationJorge, Lurdes; Dias, Teresa; Andrade, Maria; Vaz, Madalena; Dominguez, Angél; Choupina, Altino (2010). Isolation and phylogenetic analysis of two actin genes from Phytophthora cinnamomi . XVII Congresso Nacional de Bioquímica. Portopor
dc.identifier.urihttp://hdl.handle.net/10198/3389
dc.language.isoengpor
dc.peerreviewedyespor
dc.subjectPhytophthora cinnamomipor
dc.subjectInk diseasepor
dc.subjectCytoskeletonpor
dc.subjectActinpor
dc.titleIsolation and phylogenetic analysis of two actin genes from Phytophthora cinnamomipor
dc.typeconference object
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-AAM%2F67628%2F2006/PT
oaire.citation.conferencePlacePortopor
oaire.citation.endPage260por
oaire.citation.startPage259por
oaire.citation.titleXVII Congresso Nacional de Bioquímicapor
oaire.fundingStream3599-PPCDT
person.familyNameDias
person.familyNameChoupina
person.givenNameTeresa
person.givenNameAltino
person.identifier142703
person.identifier587972
person.identifier.ciencia-id1A14-77FC-9656
person.identifier.orcid0000-0002-9419-9561
person.identifier.orcid0000-0002-3956-9398
person.identifier.ridL-5382-2014
person.identifier.scopus-author-id56830642600
person.identifier.scopus-author-id14051602500
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typeconferenceObjectpor
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