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Advisor(s)
Abstract(s)
Infection by Human Papillomavirus (HPV) is the main cause of cervical cancer, highlighting the importance
of studying compounds that may reduce viral activity and its lesions/symptoms. The aromatic herb
spearmint (Mentha spicata) (MS) has proven anti-tumour properties 1. Thus, this study aimed to evaluate
the effects of an hydroethanolic extract obtained from spearmint in HPV16-transgenic (HPV+/-)
mice. The extract was obtained through maceration with ethanol/water (80:20, v/v), and the phenolic
composition was determined through HPLC-DAD-ESI/MS. A total of thirteen
compounds were identified in the hydroethanolic extract, being salvianolic acid B, rosmarinic acid and
luteolin-7-O-glucuronide, the main compounds found. Moreover, the compounds revealed to be stable in
the drinking water during the 5 tested days. Results show that HPV+/- (GIV, V and VI) have lower weight
but higher water and food intake, as expected. Analysis of HPV+/- mice GIV showed a significant increase
of superoxide dismutase activity when compared with GV (p=0.0029) and VI (p=0.0011). This suggests
that group IV was subjected to a higher oxidative stress, as expected1. These results could also mean that
MS is responsible for a decrease in the oxidative stress known to be induced by HPV, which in turn decreases
SOD, further the role of MS as an antioxidant. Regarding genetic damage, no statistically significant changes
between groups were found in the comet and micronucleus assays, implying that spearmint has no influence
on genotoxicity at the concentrations employed. There were no significant differences concerning basal
DNA damage between WT and transgenic individuals as seen in other studies 2. Further studies are required
to clarify the antioxidant and antigenotoxic effects of spearmint.
Description
Keywords
Spearmint (Mentha spicata)
Pedagogical Context
Citation
Jesus, T.M.; Silva-Reis, Rita; Pires, Maria João; Lanzarin, Germano; Félix, Luís; Venâncio, Carlos; Costa, Rui Miguel Gil; Bastos, Margarida M.S.M.; Medeiros, Rui; Nogueira, António José M.; Barros, Lillian; Ferreira, Isabel C.F.R.; Rosa, Eduardo; Oliveira, Paula A. (2021). The anti-tumour effect of spearmint (Mentha spicata) in a HPV-16-transgenic mouse model. In Lillian Barros; Bruno Melgar Castañeda; Carlos Seiti Hurtado Shiraishi (Eds.) 1st Natural products application: Health, Cosmetic and Food: book of Abstracts. Bragança
Publisher
Instituto Politécnico de Bragança