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Expression analysis by RT-PCR of GIP gene from Phytophthora cinnamomi

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dc.contributor.authorBelo, Hélio
dc.contributor.authorMartins, Fátima
dc.contributor.authorJorge, Lurdes
dc.contributor.authorSousa, Maria João
dc.contributor.authorRodrigues, Luciano
dc.contributor.authorChoupina, Altino
dc.date.accessioned2011-02-14T11:06:48Z
dc.date.available2011-02-14T11:06:48Z
dc.date.issued2010
dc.description.abstractSpecies of the genus Phytophthora secrete glucanase inhibitor proteins (GIPs) to inhibit the activity of enzymes involved in plant defense responses, including during plant infection process of Castanea sativa Mill by Phytophthora cinnamomi. GIPs show structural homology to the chymotrypsin class of serine proteases (SP) but lack proteolytic activity due to the absence of an intact catalytic triad and, thus, belong to a broader class of proteins called serine protease homologs (SPH) nonfunctional because one or more residues of the essential catalytic triad is absent (His-Asp-Ser). GIPs show high homology to the S1A subfamily of SP, however questions remain about the expression patterns and potential roles of different GIPs during pathogenesis and their possible interaction with host EGases in the plant apoplast. ORF of GIP gene from P. cinnamomi encodes a 269 aa protein. In order to understand its function, we proceeded to the heterologous expression in Pichia pastoris. The expression was studied during growth in different carbon sources and a time course of glucanase inhibitor protein production by RT-PCR was also performed. The major expression levels occurred at the medium with glucose as carbon source.por
dc.description.sponsorshipProject “Identification, characterization and role of molecular factors associated with the mechanisms of infection of Fagaceae species by Phytophthora cinnamomi” (PTDC/AGR-AAM/67628/2006) FCT
dc.identifier.citationBelo, Hélio; Martins, Fátima; Jorge, Lurdes; Sousa, Maria João; Rodrigues, Luciano; Choupina, Altino (2010). Expression analysis by RT-PCR of GIP gene from Phytophthora cinnamomi. In 9th Conference of the European Foundation for Plant Pathology. 6th Congress of the Sociedade Portuguesa de Fitopatologia. Évorapor
dc.identifier.urihttp://hdl.handle.net/10198/3378
dc.language.isoengpor
dc.peerreviewedyespor
dc.subjectCastanea sativa Mill.por
dc.subjectGlucanase inhibitor proteinspor
dc.subjectPhytophthora cinnamomipor
dc.titleExpression analysis by RT-PCR of GIP gene from Phytophthora cinnamomipor
dc.typeconference object
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-AAM%2F67628%2F2006/PT
oaire.citation.conferencePlaceÉvorapor
oaire.citation.endPage183por
oaire.citation.startPage183por
oaire.citation.title9th Conference of the European Foundation for Plant Pathology. 6th Congress of the Sociedade Portuguesa de Fitopatologiapor
oaire.fundingStream3599-PPCDT
person.familyNameSousa
person.familyNameChoupina
person.givenNameMaria João
person.givenNameAltino
person.identifier587972
person.identifier.ciencia-idD91E-3571-3979
person.identifier.ciencia-id1A14-77FC-9656
person.identifier.orcid0000-0002-9946-4926
person.identifier.orcid0000-0002-3956-9398
person.identifier.ridL-5382-2014
person.identifier.scopus-author-id55230282700
person.identifier.scopus-author-id14051602500
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typeconferenceObjectpor
relation.isAuthorOfPublicationda5c7615-9844-4fdf-b4d4-64d0c8e72de0
relation.isAuthorOfPublication314b895b-a5e2-4a92-9bc6-dd708c819be4
relation.isAuthorOfPublication.latestForDiscovery314b895b-a5e2-4a92-9bc6-dd708c819be4
relation.isProjectOfPublication5169eaef-93f4-4316-9542-d2a31ebb44d8
relation.isProjectOfPublication.latestForDiscovery5169eaef-93f4-4316-9542-d2a31ebb44d8

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