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Distribution of pyrethroid and amitraz resistance in Varroa destructor mites from honey bee (Apis mellifera) colonies in Portugal

2025Documento de conferência Acesso aberto
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datacite.subject.fosCiências Naturais::Ciências da Terra e do Ambiente
datacite.subject.sdg02:Erradicar a Fome
datacite.subject.sdg15:Proteger a Vida Terrestre
datacite.subject.sdg12:Produção e Consumo Sustentáveis
dc.contributor.authorCosta, Maíra
dc.contributor.authorYadró Garcia, Carlos A.
dc.contributor.authorRodrigues, Cláudia
dc.contributor.authorLopes, Ana Rita
dc.contributor.authorPérez-Pérez, Antonio
dc.contributor.authorMartín Hernández, Raquel
dc.contributor.authorHiges, Mariano
dc.contributor.authorPinto, M. Alice
dc.contributor.authorHenriques, Dora
dc.date.accessioned2026-02-12T14:03:19Z
dc.date.available2026-02-12T14:03:19Z
dc.date.issued2025
dc.description.abstractHoney bees (Apis mellifera) are widely used for pollination and honey production worldwide. However, they face a serious threat from the ectoparasitic mite Varroa destructor. This parasite causes varroosis and transmits multiple viruses, such as the Deformed wing virus (DWVV), compromising the health and survival of colonies. While there are many acaricides commercially available to treat colonies against varroa, the recurrent use of certain molecules is leading to the development of varroa resistance. In Portugal, two classes of synthetic compounds are currently used: pyrethroids (fluvalinate and flumethrin) and formamidines (amitraz). However, the excessive and repeated use of acaricides has led to the development of resistance in mite populations. Pyrethroid resistance is linked to mutations in the Voltage-Gated Sodium Channel (VGSC) gene, with amino acid substitutions, at position 925 and 918. Amitraz resistance is associated with mutations in the Octopamine-like β-adrenergic receptor (Octβ2R). The amino acid changes include asparagine (N) to serine (S) at position 87 (N87S), observed in France; tyrosine (Y) to histidine (H) at position 215 (Y215H), in the USA; phenylalanine (F) to leucine (L) at position 290 (F290L), in Spain; and tyrosine (Y) to phenylalanine (F) at position 337 (Y337F), in Turkey. The distribution of resistance alleles has not yet been explored in Portugal. To fill this data gap, Varroa mites from honey bee colonies across various regions of the country were analyzed through DNA extraction, PCR with specific primers, and Sanger sequencing. The results confirmed the of widespread distribution of resistance alleles to pyrethroids at positions 925 and 918, like those observed in Spain. In contrast, preliminary results did not detect any amitraz resistance alleles, although the small sample size warrants caution in interpretation. This study represents an important step in understanding V. destructor resistance to chemical treatments in Portugal, providing essential data for monitoring acaricide resistance and supporting the development of more effective management strategies to help beekeepers better protect their colonies.eng
dc.description.sponsorshipThis work was supported by the project “MITE” funded by National Beekeeping Programme 2023-2027. FCT/MCTES (PIDDAC): CIMO, UIDB/00690/2020 (DOI: 10.54499/UIDB/00690/2020); and SusTEC, LA/P/0007/2020 (DOI: 10.54499/LA/P/0007/2020).
dc.identifier.citationCosta, Maíra; Ariel Yadró, Carlos; Yadró Garcia, Carlos A.; Rodrigues, Cláudia; Lopes, Ana Rita; Lopes, Ana Rita; Pérez-Pérez, Antonio; Martín Hernández, Raquel; Higes, Mariano; Pinto, M. Alice; Pinto, M. Alice; Henriques, Dora; Henriques, Dora (2025). Distribution of pyrethroid and amitraz resistance in Varroa destructor mites from honey bee (Apis mellifera) colonies in Portugal. In Apimondia 2025, p. 841.
dc.identifier.urihttp://hdl.handle.net/10198/35731
dc.language.isoeng
dc.peerreviewedyes
dc.relationMountain Research Center
dc.relationAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectApis mellifera
dc.subjectVarroa destructor
dc.titleDistribution of pyrethroid and amitraz resistance in Varroa destructor mites from honey bee (Apis mellifera) colonies in Portugaleng
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardTitleAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0007%2F2020/PT
oaire.citation.conferencePlaceCopenhagen, Denmark
oaire.citation.titleAPIMONDIA
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameCosta
person.familyNameYadró Garcia
person.familyNameLopes
person.familyNamePinto
person.familyNameHenriques
person.givenNameMaíra
person.givenNameCarlos A.
person.givenNameAna Rita
person.givenNameM. Alice
person.givenNameDora
person.identifier.ciencia-idFB10-3DFA-D593
person.identifier.ciencia-idF71F-B08E-EC39
person.identifier.ciencia-idF014-6835-054D
person.identifier.ciencia-idF814-A1D0-8318
person.identifier.ciencia-id291F-986F-07DA
person.identifier.orcid0000-0001-6697-6581
person.identifier.orcid0000-0002-6916-3647
person.identifier.orcid0000-0002-1888-2262
person.identifier.orcid0000-0001-9663-8399
person.identifier.orcid0000-0001-7530-682X
person.identifier.scopus-author-id8085507800
person.identifier.scopus-author-id55761737300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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