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DNA extraction from plant food supplements: Influence of different pharmaceutical excipients

2015Journal article Open access
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dc.contributor.authorCosta, Joana
dc.contributor.authorAmaral, Joana S.
dc.contributor.authorFernandes, Telmo J.R.
dc.contributor.authorBatista, Andreia
dc.contributor.authorOliveira, Beatriz
dc.contributor.authorMafra, Isabel
dc.date.accessioned2018-02-19T10:00:00Z
dc.date.accessioned2018-03-01T16:04:42Z
dc.date.accessioned2018-03-05T09:18:55Z
dc.date.available2018-01-19T10:00:00Z
dc.date.available2018-03-01T16:04:42Z
dc.date.available2018-03-05T09:18:55Z
dc.date.issued2015
dc.description.abstractThe consumption of plant food supplements (PFS) has been growing globally, with an increase of misleading labeling and fraudulent practices also being reported. Recently, the use of molecular biology techniques has been proposed to detect botanical adulterations, one of the possible frauds in PFS. However, difficulties in recovering DNA from some PFS samples have been described. Aiming at using DNA-based methods for the unequivocal identification of plant species in PFS, adequate DNA isolation is required. However, PFS often contain pharmaceutical excipients known to have adsorbent properties that might interfere with DNA extraction. Thus, the aim of this work was to assess the effect of different excipients (talc, silica, iron oxide and titanium dioxide) on the recovery/amplification of DNA. For that purpose, known amounts of template maize DNA were spiked either to PFS or to model mixtures of excipients and quantified by real-time PCR. The tested excipients evidenced clear adsorption phenomena that justify the hampering effect on DNA extraction from PFS. The use of either 10% talc or 0.5% dyes completely adsorbed DNA, resulting in negative PCR amplifications. For the first time, pharmaceutical excipients were shown to affect DNA extraction explaining the inability of recovering DNA from some PFS samples in previous studies.en_EN
dc.description.sponsorshipThis work received financial support from the European Union (FEDER funds through COMPETE) and National Funds (FCT, Fundaç~ao para a Ci^encia e Tecnologia) through project EXPL/DTPSAP/ 1438/2013 (Safety of plant food supplements: searching for adulterant pharmaceutical drugs and plants) and UID/QUI/50006/ 2013. Joana Costa and Telmo J.R. Fernandes are grateful to FCT grants SFRH/BPD/102404/2014 and SFRH/BD/93711/2013, respectively, financed by POPH-QREN (subsidized by FSE and MCTES).
dc.description.sponsorshipThis work received financial support from the European Union (FEDER funds through COMPETE) and National Funds (FCT, Fundaç~ao para a Ci^encia e Tecnologia) through project EXPL/DTPSAP/ 1438/2013 (Safety of plant food supplements: searching for adulterant pharmaceutical drugs and plants) and UID/QUI/50006/ 2013. Joana Costa and Telmo J.R. Fernandes are grateful to FCT grants SFRH/BPD/102404/2014 and SFRH/BD/93711/2013, respectively, financed by POPH-QREN (subsidized by FSE and MCTES).
dc.description.versioninfo:eu-repo/semantics/publishedVersionen_EN
dc.identifier.citationCosta, Joana; Amaral, Joana S.; Fernandes, Telmo J.R.; Batista, Andreia; Oliveira, M. Beatriz P.P.; Mafra, Isabel (2015). DNA extraction from plant food supplements: Influence of different pharmaceutical excipients. Molecular and Cellular Probes. ISSN 0890-8508. 29, p. 473-478en_EN
dc.identifier.doi10.1016/j.mcp.2015.06.002en_EN
dc.identifier.issn0890-8508
dc.identifier.urihttp://hdl.handle.net/10198/16098
dc.language.isoeng
dc.peerreviewedyesen_EN
dc.relationStructural features and immunoreactivity of plant food allergens: impact of technological food processing and in vitro digestibility
dc.subjectDNA adsorptionen_EN
dc.subjectDNA extractionen_EN
dc.subjectPharmaceutical excipientsen_EN
dc.subjectPlant food supplementsen_EN
dc.subjectReal-time PCRen_EN
dc.titleDNA extraction from plant food supplements: Influence of different pharmaceutical excipientsen_EN
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStructural features and immunoreactivity of plant food allergens: impact of technological food processing and in vitro digestibility
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/COMPETE/EXPL%2FDTP-SAP%2F1438%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F102404%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F93711%2F2013/PT
oaire.fundingStreamCOMPETE
oaire.fundingStream5876
oaire.fundingStreamSFRH
person.familyNameAmaral
person.givenNameJoana S.
person.identifier.ciencia-id5319-7DE8-BEDA
person.identifier.orcid0000-0002-3648-7303
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccessen_EN
rcaap.typearticleen_EN
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relation.isAuthorOfPublication.latestForDiscovery42be2cf4-adc4-4e7f-ac60-7aab515b38cd
relation.isProjectOfPublication730054a0-eed4-4013-806b-672d1d27b87d
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