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Optimization of ultrasound assisted extraction of ergosterol from Agaricus bisporus L

dc.contributor.authorHeleno, Sandrina A.
dc.contributor.authorDiz, Patrícia do Carmo Claro
dc.contributor.authorBarros, Lillian
dc.contributor.authorPais, Luís S.
dc.contributor.authorRodrigues, Alírio
dc.contributor.authorBarreiro, M.F.
dc.contributor.authorFerreira, Isabel C.F.R.
dc.date.accessioned2015-11-11T14:53:58Z
dc.date.available2015-11-11T14:53:58Z
dc.date.issued2015
dc.description.abstractMushrooms are appreciated worldwide, not only for their nutritional value and exquisite flavor and texture, but also for their medicinal properties. There is scientific evidence demonstrating the benefits of mushrooms ingestion due to their richness in bioactive compounds such as mycosterols, in particular ergosterol [1]. Agaricus bisporus L. is the most consumed mushroom worldwide presenting a high content of ergosterol, which represents 90% of its sterol fraction [2]. Thus, it is an interesting matrix to obtain ergosterol, a molecule with a high commercial value. According to literature, ergosterol concentration can vary between 3 and 9 mg per g of dried mushroom. Traditional methods such as maceration and soxhlet extraction are being replaced by emerging methodologies such as ultrasound assisted extraction in order to decrease the used solvent amount, the extraction time and, of course, increasing the extraction yield [3]. In the present work, A. bisporus was extraction solvent type (hexane and ethanol), ultrasound amplitude (50%, 75% and 100%) and sonication time (5 min, 10 min and 15 min). Moreover, in order to decrease the process complexity, it was evaluated the pertinence of the saponification step. Ethanol proved to be the best solvent to extract higher levels of ergosterol (671.5 ± 0.5 mg/100 g dw, at 75% amplitude for 15 min), once hexane was only able to extract 152.2 ± 0.2 mg/100 g dw, in the same conditions. Nevertheless, the hexane extract showed higher purity (11%) when compared with the ethanol counterpart (4%). Furthermore, in the case of the ethanolic extract, the saponification step increased its purity to 21%, while for the hexane extract the purity was similar; in fact, hexane presents higher selectivity for the lipophilic compounds comparatively with ethanol. Overall, ultrasound assisted extraction proved to be an efficient technology to maximize ergosterol extraction yield, but other emerging technologies such as microwave assisted extraction can be applied with foreseen interesting results.pt_PT
dc.identifier.citationHeleno, Sandrina A.; Diz, Patricia; Barros, Lillian; Pais, L.S.; Rodrigues, A.E.; Barreiro, M.F.; Ferreira, Isabel C.F.R. (2015). Optimization of ultrasound assisted extraction of ergosterol from Agaricus bisporus L. In EuroFoodChem XVIII International Conference 2015. Madridpt_PT
dc.identifier.urihttp://hdl.handle.net/10198/12324
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationNORTE-07-0124-FEDER-000014pt_PT
dc.relationNORTE-07-0162-FEDER-000050pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAgaricus bisporuspt_PT
dc.subjectErgosterolpt_PT
dc.subjectUltrasound assisted extractionpt_PT
dc.titleOptimization of ultrasound assisted extraction of ergosterol from Agaricus bisporus Lpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/PEst-OE%2FAGR%2FUI0690%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FEQU%2F50020%2F2013/PT
oaire.citation.titleEuroFoodChem XVIII International Conference 2015pt_PT
oaire.fundingStream5876
oaire.fundingStream5876
person.familyNameHeleno
person.familyNameBarros
person.familyNamePais
person.familyNameBarreiro
person.familyNameFerreira
person.givenNameSandrina A.
person.givenNameLillian
person.givenNameLuís S.
person.givenNameFilomena
person.givenNameIsabel C.F.R.
person.identifier469085
person.identifier144781
person.identifier.ciencia-id8B18-3095-6FC9
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person.identifier.orcid0000-0001-7224-1098
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0002-3000-2862
person.identifier.orcid0000-0002-6844-333X
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridL-5951-2014
person.identifier.ridJ-3600-2013
person.identifier.ridL-9802-2014
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id30067731800
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id6603930478
person.identifier.scopus-author-id57188719717
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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