Publication
Optimization of the aptamers’ immobilization conditions for maximizing the response of a dualaptasensor for cancer biomarker detection
| dc.contributor.author | Meirinho, Sofia G. | |
| dc.contributor.author | Ezziddine, Maha | |
| dc.contributor.author | Veloso, Ana C.A. | |
| dc.contributor.author | Dias, L.G. | |
| dc.contributor.author | Rodrigues, Lígia R. | |
| dc.contributor.author | Othmane, Ali | |
| dc.contributor.author | Peres, António M. | |
| dc.date.accessioned | 2020-02-27T15:02:04Z | |
| dc.date.available | 2020-02-27T15:02:04Z | |
| dc.date.issued | 2017 | |
| dc.description.abstract | A simple and sensitive method that allows the simultaneous detection of single or multiple cancer biomarkers is envisaged and may be an important tool in cancer diagnosis. Osteopontin (OPN) is a protein that is present in several body fluids and has been reported as a possible cancer biomarker. The voltammetric signals generated by the dual-aptasensor array, after the formation of the aptamers-protein complex, were monitored using cyclic voltammetry (CV) and square-wave voltammetry (SWV), using [Fe(CN)6] 3/ 4 as a redox probe. The aim of this work is to optimize the experimental conditions to development a label-free voltammetric dual-aptasensor array for the detection of human osteopontin using a 2k factorial experimental design. | pt_PT |
| dc.description.sponsorship | This work was financially supported by Project POCI-01 0145-FEDER-006984 Associate Laboratory LSRE-LCM, Project UID/QUI/00616/2013 CQ-VR, and of UID/BIO/04469/2013 - CEB all funded by FEDER - Fundo Europeu de Desenvolvimento Regional through COMPETE - Programa Operacional Competitividade e Internacionalização (POCI) and by national funds through FCT - o para a ncia e a Tecnologia, Portugal. S.G. Meirinho also acknowledges the research grant provided by Project UID/EQU/50020/2013. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Meirinho, Sofia G.; Ezzedine, Maha; Veloso, Ana C.A.; Dias, L.G.; Rodrigues, Lígia R.; Othmane, Ali; Peres, António M. (2017). Optimization of the aptamers’ immobilization conditions for maximizing the response of a dualaptasensor for cancer biomarker detection. In XIV Reunión Ibernam. Tordesillas | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10198/20731 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | pt_PT |
| dc.title | Optimization of the aptamers’ immobilization conditions for maximizing the response of a dualaptasensor for cancer biomarker detection | pt_PT |
| dc.type | conference poster | |
| dspace.entity.type | Publication | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F00616%2F2013/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876/UID%2FBIO%2F04469%2F2013/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876/UID%2FEQU%2F50020%2F2013/PT | |
| oaire.citation.conferencePlace | Valladolid, Espanha | pt_PT |
| oaire.citation.title | XIV Reunión IberNAM | pt_PT |
| oaire.fundingStream | 5876 | |
| oaire.fundingStream | 5876 | |
| oaire.fundingStream | 5876 | |
| person.familyName | Dias | |
| person.familyName | Peres | |
| person.givenName | Luís G. | |
| person.givenName | António M. | |
| person.identifier | 107333 | |
| person.identifier.ciencia-id | 2F11-9092-FAAF | |
| person.identifier.ciencia-id | CF16-5443-F420 | |
| person.identifier.orcid | 0000-0002-1210-4259 | |
| person.identifier.orcid | 0000-0001-6595-9165 | |
| person.identifier.rid | I-8470-2012 | |
| person.identifier.scopus-author-id | 23569169900 | |
| person.identifier.scopus-author-id | 7102331969 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | conferenceObject | pt_PT |
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