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Antimicrobial and cytotoxic activities of Alnus rugosa L. aerial partsand identification of the bioactive components

2014Journal article Open access
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dc.contributor.authorRashed, Khaled N.
dc.contributor.authorĆirić, Ana
dc.contributor.authorGlamočlija, Jasmina
dc.contributor.authorCalhelha, Ricardo C.
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorSoković, Marina
dc.date.accessioned2014-08-14T09:35:37Z
dc.date.available2014-08-14T09:35:37Z
dc.date.issued2014
dc.description.abstractThis present investigation was undertaken to study antimicrobial and cytotoxic activities ofdichloromethane, ethyl acetate, and methanol 80% extracts from Alnus rugosa L. aerial parts, as wellinvestigate the bio-active compounds of each extract. Antimicrobial activity of the mentioned extractsagainst various bacteria and fungi using microdilution method, and cytotoxicity against five differenthuman tumor cell lines, and in a non-tumor liver cells primary culture were tested. Extracts were sub-jected to phytochemical analysis and column chromatography eluted with different solvents, n-hexane,dichloromethane, ethyl acetate, and methanol gradually. The results showed that the extracts had asignificant antimicrobial activity against all the tested bacteria and fungi, and proved a significant cyto-toxic effect. Methanol 80% extract exhibited the highest antibacterial effect, and ethyl acetate extractwas the most active as antifungal agent while dichloromethane extract showed the lowest antifungaleffect. Dichloromethane extract was the most active against all the tested tumor cell lines. Chromato-graphic separation of dichloromethane extract of A. rugosa aerial parts resulted in the isolation andidentification of -sitosterol, -sitosterol 3-O- -glucoside and apigenin, while from ethyl acetate extract,diosmetin, naringenin, luteolin, and luteolin 7-O- -glucoside were identified, and from methanol 80%extract, quercetin 3-O- -rhamnoside, isorhamnetin 3-O- -glucoside and isorhamnetin 3-O- -glucoside7-O- -rhamnoside were identified. In conclusion, the present research provided significant informationabout antimicrobial and cytotoxic activities and compounds isolated from A. rugosa aerial parts extracts.por
dc.description.sponsorshipPEst-OE/AGR/UI0690/2011 for financial support to CIMO. R.Calhelha thanks to FCT, POPH-QREN and FSE for his grant (SFRH/BD/78307/2011), and Serbian Ministry of Education, Sci-ence and Technological Development, grant number 173032 forfinancial support.por
dc.identifier.citationRashed, Khaled N.; Ciric, Ana; Glamočlija, Jasmina; Calhelha, Ricardo C.; Ferrreira, Isabel C.F.R.; Sokovic, Marina (2014). Antimicrobial and cytotoxic activities of Alnus rugosa L. aerial partsand identification of the bioactive components. Industrial Crops and Products. ISSN 0926-6690. 59, p. 189–196por
dc.identifier.doi0.1016/j.indcrop.2014.05.017
dc.identifier.issn0926-6690
dc.identifier.urihttp://hdl.handle.net/10198/10155
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relationStrategic Project - UI 690 - 2011-2012
dc.subjectAlnus rugosapor
dc.subjectAntibacterial activitypor
dc.subjectAntifungal activitypor
dc.subjectAnticancer activitypor
dc.subjectNatural drugspor
dc.subjectPhytochemicalspor
dc.titleAntimicrobial and cytotoxic activities of Alnus rugosa L. aerial partsand identification of the bioactive componentspor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 690 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FAGR%2FUI0690%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F78307%2F2011/PT
oaire.citation.endPage196por
oaire.citation.startPage189por
oaire.citation.titleIndustrial Crops and Productspor
oaire.citation.volume196por
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamSFRH
person.familyNameCalhelha
person.familyNameFerreira
person.givenNameRicardo C.
person.givenNameIsabel C.F.R.
person.identifier144781
person.identifier.ciencia-idF313-E3CE-554E
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0002-6801-4578
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridJ-2172-2014
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id6507978333
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typearticlepor
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relation.isAuthorOfPublicationbd0d1537-2e03-41fb-b27a-140af9c35db8
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