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Optimized analysis of organic acids in edible mushrooms from Portugal by ultra fast liquid chromatography and photodiode array detection

2013Journal article Open access
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dc.contributor.authorBarros, Lillian
dc.contributor.authorPereira, Carla
dc.contributor.authorFerreira, Isabel C.F.R.
dc.date.accessioned2013-07-23T17:05:24Z
dc.date.available2013-07-23T17:05:24Z
dc.date.issued2013
dc.description.abstractOrganic acid profiles of different mushroom species were obtained by ultra-fast liquid chromatography (UFLC), by means of photodiode array detector (PDA). The chromatographic separation was achieved using a SphereClone (Phenomenex) reverse phase C18 column using an isocratic elution with sulphuric acid (3.6 mM) at a flow rate of 0.8 mL/min. All the compounds were separated in 8 min. The method was optimized using Agaricus bisporus sample and proved to be reproducible and accurate. Organic acid profiles were quite homogeneous for all mushroom samples; oxalic, malic and fumaric acids were the main organic acids; some samples also presented quinic and citric acids. Sarcondon imbricatus was the species that presented the highest total content (254.09 mg/g dw), while Bovista nigrescens presented the lowest concentration (1.33 mg/g dw). The high amounts of organic acids present in all the species may suggest that they could be related to the antioxidant activity found in these species and previously reported by us.por
dc.description.sponsorshipFundação para a Ciência e a Tecnologia (FCT, Portugal) and COMPETE/QREN/EU for financial support to this work (research project PTDC/AGR-ALI/110062/2009) and to CIMO (strategic project PEst-OE/AGR/UI0690/2011). L. Barros also thanks to FCT, POPH-QREN and FSE for her grant (SFRH/BPD/4609/2008).por
dc.identifier.citationBarros, Lillian; Pereira, Carla; Ferreira, Isabel C.F.R. (2013). Optimized analysis of organic acids in edible mushrooms from Portugal by ultra fast liquid chromatography and photodiode array detection. Food Analytical Methods. ISSN 1936-9751. 6:1, p. 309-316por
dc.identifier.issn1936-9751
dc.identifier.urihttp://hdl.handle.net/10198/8536
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringerpor
dc.relationPortuguese Wild Mushrooms: Chemical characterization and functional study of antiproliferative and proapoptotic properties in cancer cell lines
dc.relationStrategic Project - UI 690 - 2011-2012
dc.subjectEdible mushroomspor
dc.subjectUFLC-PADpor
dc.subjectAnalysis optimizationpor
dc.subjectOrganic acidspor
dc.titleOptimized analysis of organic acids in edible mushrooms from Portugal by ultra fast liquid chromatography and photodiode array detectionpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlePortuguese Wild Mushrooms: Chemical characterization and functional study of antiproliferative and proapoptotic properties in cancer cell lines
oaire.awardTitleStrategic Project - UI 690 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FAGR-ALI%2F110062%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FAGR%2FUI0690%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F44609%2F2008/PT
oaire.citation.endPage316por
oaire.citation.issue1por
oaire.citation.startPage309por
oaire.citation.titleFood Analytical Methodspor
oaire.citation.volume6por
oaire.fundingStream5876-PPCDTI
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamSFRH
person.familyNameBarros
person.familyNamePereira
person.familyNameFerreira
person.givenNameLillian
person.givenNameCarla
person.givenNameIsabel C.F.R.
person.identifier469085
person.identifier1415151
person.identifier144781
person.identifier.ciencia-id9616-35CB-D001
person.identifier.ciencia-idEF10-2739-2B70
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0003-0093-771X
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridJ-3600-2013
person.identifier.ridK-1629-2016
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id55627876424
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typearticlepor
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