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Differences in the endophytic microbiome of olive cultivars infected by xylella fastidiosa across seasons

2020Journal article Open access
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dc.contributor.authorGiampetruzzi, Annalisa
dc.contributor.authorBaptista, Paula
dc.contributor.authorMorelli, Massimiliano
dc.contributor.authorCameirão, Cristina
dc.contributor.authorNeto, Teresa Lino
dc.contributor.authorCosta, Daniela
dc.contributor.authorD’Attoma, Giusy
dc.contributor.authorKubaa, Raied Abou
dc.contributor.authorAltamura, Giuseppe
dc.contributor.authorSaponari, Maria
dc.contributor.authorPereira, J.A.
dc.contributor.authorSaldarelli, Pasquale
dc.date.accessioned2018-02-19T10:00:00Z
dc.date.accessioned2021-02-27T16:30:30Z
dc.date.available2018-01-19T10:00:00Z
dc.date.available2021-02-27T16:30:30Z
dc.date.issued2020
dc.description.abstractThe dynamics of Xylella fastidiosa infections in the context of the endophytic microbiome was studied in field-grown plants of the susceptible and resistant olive cultivars Kalamata and FS17. Whole metagenome shotgun sequencing (WMSS) coupled with 16S/ITS rRNA gene sequencing was carried out on the same trees at two different stages of the infections: In Spring 2017 when plants were almost symptomless and in Autumn 2018 when the trees of the susceptible cultivar clearly showed desiccations. The progression of the infections detected in both cultivars clearly unraveled that Xylella tends to occupy the whole ecological niche and suppresses the diversity of the endophytic microbiome. However, this trend was mitigated in the resistant cultivar FS17, harboring lower population sizes and therefore lower Xylella average abundance ratio over total bacteria, and a higher α-diversity. Host cultivar had a negligible effect on the community composition and no clear associations of a single taxon or microbial consortia with the resistance cultivar were found with both sequencing approaches, suggesting that the mechanisms of resistance likely reside on factors that are independent of the microbiome structure. Overall, Proteobacteria, Actinobacteria, Firmicutes, and Bacteriodetes dominated the bacterial microbiome while Ascomycota and Basidiomycota those of Fungi.en_EN
dc.description.sponsorshipThis research was funded by the EU H2020 Research Project XF-ACTORS “Xylella Fastidiosa Active Containment Through a multidisciplinary-Oriented Research Strategy” (Grant Agreement 727987) and by the Mountain Research Center—CIMO (UIDB/00690/2020) and BioISI (UIDB/04046/2020). The Funding Agencies are not responsible for any use that may be made of the information it contains.
dc.description.versioninfo:eu-repo/semantics/publishedVersionen_EN
dc.identifier.citationGiampetruzzi, Annalisa; Baptista, Paula; Morelli, Massimiliano; Cameirão, Cristina; Neto, Teresa Lino; Costa, Daniela; D’attoma, Giusy; Kubaa, Raied Abou; Altamura, Giuseppe; Saponari, Maria; Pereira, José Alberto; Saldarelli, Pasquale (2020). Differences in the endophytic microbiome of olive cultivars infected by xylella fastidiosa across seasons. Pathogens. ISSN 2076-0817. 9:9, p. 1-29en_EN
dc.identifier.doi10.3390/pathogens9090723en_EN
dc.identifier.urihttp://hdl.handle.net/10198/23389
dc.language.isoeng
dc.peerreviewedyesen_EN
dc.relationBiosystems and Integrative Sciences Institute
dc.subject16S/ITS sequencingen_EN
dc.subjectArchaeaen_EN
dc.subjectBacteriaen_EN
dc.subjectFS17en_EN
dc.subjectFungien_EN
dc.subjectKalamataen_EN
dc.subjectResistanceen_EN
dc.subjectShotgun metagenomic sequencingen_EN
dc.subjectXylella fastidiosaen_EN
dc.titleDifferences in the endophytic microbiome of olive cultivars infected by xylella fastidiosa across seasonsen_EN
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBiosystems and Integrative Sciences Institute
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04046%2F2020/PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameBaptista
person.familyNameCameirão
person.familyNamePereira
person.givenNamePaula
person.givenNameCristina
person.givenNameJosé Alberto
person.identifier.ciencia-id7D11-FE1E-CD0F
person.identifier.ciencia-id9511-984B-7350
person.identifier.ciencia-id611F-80B2-A7C1
person.identifier.orcid0000-0001-6331-3731
person.identifier.orcid0000-0001-5745-9822
person.identifier.orcid0000-0002-2260-0600
person.identifier.ridL-6798-2014
person.identifier.scopus-author-id14051688000
person.identifier.scopus-author-id57204366348
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccessen_EN
rcaap.typearticleen_EN
relation.isAuthorOfPublication3f35226a-b17a-4f7d-8da1-3297105cbfe9
relation.isAuthorOfPublicationc7e28508-95c5-4441-a2c3-91d69059016d
relation.isAuthorOfPublication7932162e-a2da-4913-b00d-17babbe51857
relation.isAuthorOfPublication.latestForDiscovery3f35226a-b17a-4f7d-8da1-3297105cbfe9
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relation.isProjectOfPublication.latestForDiscovery0ee48f8e-8986-4565-a589-0a1688fb9fd4

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