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Dietary sugars analysis: quantification of fructooligossacharides during fermentation by HPLC-RI method

2014Journal article Open access
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dc.contributor.authorCorreia, Daniela M.
dc.contributor.authorDias, L.G.
dc.contributor.authorVeloso, Ana C.A.
dc.contributor.authorDias, Teresa
dc.contributor.authorRocha, Isabel
dc.contributor.authorRodrigues, Lígia R.
dc.contributor.authorPeres, António M.
dc.date.accessioned2015-05-12T09:55:09Z
dc.date.available2015-05-12T09:55:09Z
dc.date.issued2014
dc.description.abstractIn this work, a simple chromatographic method is proposed and in-house validated for the quantification of total and individual fructooligossacharides (e.g., 1-kestose, nystose, and 1F-fructofuranosylnystose). It was shown that a high-performance liquid chromatography with refractive index detector could be used to monitor the dynamic of fructooligossacharides production via sucrose fermentation using Aspergillus aculeatus. This analytical technique may be easily implemented at laboratorial or industrial scale for fructooligossacharides mass-production monitoring allowing also controlling the main substrate (sucrose) and the secondary by-products (glucose and fructose). The proposed chromatographic method had a satisfactory intra- and inter-day variability (in general, with a relative standard deviation lower than 5%), high sensitivity for each sugar (usually, with a relative error lower than 5%), and low detection (lower than 0.06+/-0.04 g/L) and quantification (lower than 0.2+/-0.1 g/L) limits. The correct quantification of fructooligossacharides in fermentative media may allow a more precise nutritional formulation of new functional foods, since it is reported that different fructooligossacharides exhibit different biological activities and effects.por
dc.description.sponsorshipFCT and FEDER under Program COMPETE (Project PEst-C/EQB/LA0020/2013), by the Strategic Project PEst-OE/EQB/LA0023/2013 and by the project ref. RECI/BBB-EBI/0179/2012(project number FCOMP- 01-0124-FEDER-027462)por
dc.identifier.citationCorreia, Daniela; Dias, Luís G.; Veloso, Ana C.A.; Dias, Teresa; Rocha, Isabel; Rodrigues, Lígia R.; Peres, António M. (2014). Dietary sugars analysis: quantification of fructooligossacharides during fermentation by HPLC-RI method. Frontiers in Nutrition. ISSN 2296-861X. 1, p. 1-9por
dc.identifier.doihttp://dx.doi.org/10.3389/fnut.2014.00011
dc.identifier.issn2296-861X
dc.identifier.urihttp://hdl.handle.net/10198/11823
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherFrontierspor
dc.subjectDietary sugarspor
dc.subjectFructooligossacharidespor
dc.subjectHPLC-RI methodpor
dc.subjectYield fermentation processpor
dc.subjectIn-house validationpor
dc.titleDietary sugars analysis: quantification of fructooligossacharides during fermentation by HPLC-RI methodpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage9por
oaire.citation.startPage1por
oaire.citation.titleFrontiers in Nutrition - Nutrition and Food Science Technologypor
oaire.citation.volume1por
person.familyNameDias
person.familyNameDias
person.familyNamePeres
person.givenNameLuís G.
person.givenNameTeresa
person.givenNameAntónio M.
person.identifier142703
person.identifier107333
person.identifier.ciencia-id2F11-9092-FAAF
person.identifier.ciencia-idCF16-5443-F420
person.identifier.orcid0000-0002-1210-4259
person.identifier.orcid0000-0002-9419-9561
person.identifier.orcid0000-0001-6595-9165
person.identifier.ridI-8470-2012
person.identifier.scopus-author-id23569169900
person.identifier.scopus-author-id56830642600
person.identifier.scopus-author-id7102331969
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationeac8c166-4056-4ed0-8d8d-7ecb2c4481a5
relation.isAuthorOfPublication87c9cae0-314d-4622-ba85-4d3ec5726606
relation.isAuthorOfPublication7d93be47-8dc4-4413-9304-5b978773d3bb
relation.isAuthorOfPublication.latestForDiscovery87c9cae0-314d-4622-ba85-4d3ec5726606

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