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A study of the antitumour potential of three Portuguese wild mushrooms

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dc.contributor.authorVaz, Josiana A.
dc.contributor.authorFerreira, Diana G.
dc.contributor.authorAlmeida, Gabriela M.
dc.contributor.authorCardoso, Adélia
dc.contributor.authorAlmeida, Ana P.
dc.contributor.authorMartins, Anabela
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorVasconcelos, M. Helena
dc.date.accessioned2011-08-18T13:47:21Z
dc.date.available2011-08-18T13:47:21Z
dc.date.issued2011
dc.description.abstractNatural matrixes such as mushrooms represent a rich source of biologically active compounds with recognized potential in drug discovery and development [1,2]. Indeed, many pre-clinical studies have been conducted in human tumour cell lines and in some cases, a number of compounds extracted from mushrooms have entered clinical trials [3]. Our previous results showed that extracts from Agaricus arvensis, Suillus collinitus and Clytocibe alexandri are promising sources of low molecular weight bioactive compounds [4]. The aim of the present work was to study the antitumour potential of the extracts and isolated compounds from three Portuguese wild mushrooms by verifying their effect on various human tumour cell lines in what concerns effect on cell growth, cell cycle profile and programmed cell death. Wild mushrooms were collected from the Northeast of Portugal and classified as Agaricus arvensis, Suillus collinitus and Clitocybe alexandri. Phenolic (methanolic and ethanolic) and polissacharidic extracts were prepared. The effect of the extracts on tumour cell growth inhibition was verified with the SRB assay and the GI50 of each extract was determined for each of the cell lines studied (NCI-H460, MCF-7, AGS and HCT-15). Our preliminary results revealed that all the extracts from Clitocybe alexandri are capable of causing cell growth inhibition and provided GI50 concentrations bellow 60µg/ml in all the cell lines tested [4]. Regarding the effect of the Agaricus arvensis extracts, they all caused an inhibition of cell growth in all cell lines, particularly the methanolic extract which revealed to be a very potent inhibitor of cell growth, especially in the MCF-7 cell line. The evaluation of the effect of the Suillus collinitus extracts will be carried out as well as cell cycle and apoptosis analyses, by flow cytometry. Finally, the isolation and characterization of compounds from these extracts will also be carried out, using HPLC-DAD or HPLC-RI. The structures of the compounds will be established by NMR spectral analysis (1H, 13C, DEPT, COSY, HSQC and HMBC).por
dc.description.sponsorshipFCT and COMPETE/QREN/UE- project PTDC/AGR-ALI/110062/2009por
dc.identifier.citationVaz, Josiana A.; Ferreira, Diana F.; Almeida, Gabriela M.; Cardoso, Adélia; Almeida, Ana P.; Martins, Anabela; Ferreira, Isabel C.F.R.; Vasconcelos, M. Helena (2011). A study of the antitumour potential of three Portuguese wild mushrooms. In 4th Meeting of Young Researchers of U. Portopor
dc.identifier.urihttp://hdl.handle.net/10198/6002
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherUniversidade do Portopor
dc.relationPortuguese Wild Mushrooms: Chemical characterization and functional study of antiproliferative and proapoptotic properties in cancer cell lines
dc.titleA study of the antitumour potential of three Portuguese wild mushroomspor
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitlePortuguese Wild Mushrooms: Chemical characterization and functional study of antiproliferative and proapoptotic properties in cancer cell lines
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FAGR-ALI%2F110062%2F2009/PT
oaire.citation.conferencePlacePortopor
oaire.citation.titleIJUP’2011. 4th Meeting of Young Researchers of U. Porto, 17 e 18 de Fevereiro de 2011por
oaire.fundingStream5876-PPCDTI
person.familyNameVaz
person.familyNameMartins
person.familyNameFerreira
person.givenNameJosiana A.
person.givenNameAnabela
person.givenNameIsabel C.F.R.
person.identifier144781
person.identifier.ciencia-id171B-88BA-64F5
person.identifier.ciencia-id7B18-A810-6B93
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0002-6989-1169
person.identifier.orcid0000-0001-6218-4413
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridG-5488-2013
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id16305323200
person.identifier.scopus-author-id7203013518
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typeconferenceObjectpor
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