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Advisor(s)
Abstract(s)
Genetic analysis of the honey bee spermathecal content can be particularly useful to provide
an estimate of the genetic diversity and purity of the surrounding populations. Here we
compared the concentration and quality of DNA extracted from queen spermatheca using
four commercial kits to determine the best method to obtain DNA suitable for single nucleotide
polymorphism genotyping by next-generation sequencing. The four kits were tested
with different adjustments in the lysis incubation time, use of RNA-carrier, elution conditions
and number of re-elutions. Only the use of QIAamp DNA Microkit with 3 h of lysis incubation,
the addition of RNA-carrier and multiple re-elutions produced a DNA concentration
over the required threshold.
Description
Keywords
Honey bee Spermatheca Genomic DNA isolation DNA extraction
Pedagogical Context
Citation
Ezzaky, Youssef; Elmoslih, Abdelkhaleq; Silva, Beatriz Nunes; Bonilla‐Luque, Olga M.; Possas, Arícia; Valero, Antonio; Cadavez, Vasco; Gonzales‐Barron, Ursula; Achemchem, Fouad (2023). In vitro antimicrobial activity of extracts and essential oils of Cinnamomum, Salvia, and Menthaspp. against foodborne pathogens: a meta‐analysis study. Comprehensive Reviews in Food Science and Food Safety. ISSN 1541-4337. 22:6, p. 1-4
Publisher
Wiley