Non-conventional yeasts as hosts for heterologous protein production.

Yeasts are an attractive group of lower eukaryotic microorganisms, some of which are used in several industrial processes that include brewing, baking and the production of a variety of biochemical compounds. More recently, yeasts have been developed as host organisms for the production of foreign (heterologous) proteins. Saccharomyces cerevisiae has usually been the yeast of choice, but an increasing number of alternative non-Saccharomyces yeasts has now become accessible for modern molecular genetics techniques. Some of them exhibit certain favourable traits such as high-level secretion or very strong and tightly regulated promoters, offering significant advantages over traditional bakers' yeast. In the present work, the current status of Kluyveromyces lactis, Yarrowia lipolytica, Hansenula polymorpha and Pichia pastoris (the best-known alternative yeast systems) is reviewed. The advantages and limitations of these systems are discussed in relation to S. cerevisiae.

Keywords

Kluyveromyces lactis Yarrowia lipolytica Pichia pastoris Hansenula polymorpha Heterologous protein

URI

http://hdl.handle.net/10198/10929

Citation

Domínguez, A.; Fermiñán, E.; Sánchez, M.; González, Francisco; Pérez-Campo, F. M.; García, S.; Herrero, A.B.; San Vicente, A.; Cabello, J.; Prado, M.; Iglesias, F.J.; Choupina, Altino; Burguillo, F.J.; Fernández-Lago, L.; López, M.C. (1988). Non-conventional yeasts as hosts for heterologous protein production. International Microbiology. ISSN 1139-6709. 1, p. 131–142