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Antibodies to Toxoplasma gondii and Leishmania spp. in domestic cats from Luanda, Angola

2017Journal article Open access
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dc.contributor.authorLopes, Ana Patrícia
dc.contributor.authorOliveira, Ana Cristina
dc.contributor.authorGranada, Sara
dc.contributor.authorRodrigues, Filipa T.
dc.contributor.authorPapadopoulos, Elias
dc.contributor.authorSchallig, Henk
dc.contributor.authorDubey, Jitender P.
dc.contributor.authorCardoso, Luís Miguel
dc.date.accessioned2018-01-25T10:00:00Z
dc.date.accessioned2018-02-02T10:36:50Z
dc.date.available2018-01-25T10:00:00Z
dc.date.available2018-02-02T10:36:50Z
dc.date.issued2017
dc.description.abstractToxoplasma gondii and Leishmania spp. are zoonotic protozoa of importance to animal and public health. The present study aimed to assess for the first time the seroprevalence of these zoonotic parasites in a domestic feline population living in Luanda, Angola. One hundred and two cats were sampled at a veterinary medical centre, from May 2014 to February 2016. The age of the cats ranged from 2.5 to 143 months (median: 12 months; interquartile range: 7.5–24). Serum samples were tested for immunoglobulin (Ig) G antibodies to T. gondii at two-fold dilutions of 1:20 to 1:2560 with a modified agglutination test (MAT) commercial kit. The direct agglutination test (DAT) for titration of IgG antibodies specific to Leishmania spp. used a standard freeze-dried antigen at a concentration of 5 × 10 7 promastigotes per milliliter, following a predefined protocol. Two-fold dilution series ranging from 1:25 to 1:800 were tested, with a cut-off titre of 100 chosen for seropositivity. Four out of 102 cats (3.9%; 95% confidence interval [CI]: 1.1–9.7) had antibodies to T. gondii: one had a titer of 20, one a titer of 160, and two had a titer ≥ 2560. No cat (0.0%; CI: 0.0–3.5) was found seropositive for Leishmania spp. A statistically significant difference was found between T. gondii seroprevalence and Leishmania spp. seroprevalence (p = 0.043). The odds of a cat being seropositive to T. gondii increased by an average factor of 1.58 for each 1-year increase in age (p = 0.003). The sampled cats were well-cared animals and may not represent the overall feline population of Angola at the national and city levels. The fact that only 12 out of the 102 sampled cats ate or had access to raw or undercooked meat and/or viscera may have reduced the likelihood of finding seropositive results. Under these circumstances, additional studies, including a larger number of cats, are necessary for a more comprehensive assessment of the zoonotic risk posed by these animals in Angola.en_EN
dc.description.sponsorshipThe authors would like to express their gratitude to Hugo Vilhena for his logistic support. This work was sponsored by the Foundation for Science and Technology (FCT), Ministry of Education and Science, Portugal, under the Projects UID/CVT/00772/2013 and UID/CVT/ 0772/2016.
dc.description.versioninfo:eu-repo/semantics/publishedVersionen_EN
dc.identifier.citationLopes, Ana Patrícia; Oliveira, Ana Cristina; Granada, Sara; Rodrigues, Filipa T.; Papadopoulos, Elias; Schallig, Henk; Dubey, Jitender P.; Cardoso, Luís (2017). Antibodies to Toxoplasma gondii and Leishmania spp. in domestic cats from Luanda, Angola. Veterinary Parasitology. ISSN 0304-4017. 239, p. 15-18en_EN
dc.identifier.doi10.1016/j.vetpar.2017.04.009en_EN
dc.identifier.issn0304-4017
dc.identifier.urihttp://hdl.handle.net/10198/15502
dc.language.isoeng
dc.peerreviewedyesen_EN
dc.relationCentro de Ciência Animal e Veterinária
dc.subjectAngolaen_EN
dc.subjectAntibodiesen_EN
dc.subjectCat felineen_EN
dc.subjectLeishmaniaen_EN
dc.subjectLuandaen_EN
dc.subjectSeroprevalenceen_EN
dc.subjectToxoplasmaen_EN
dc.titleAntibodies to Toxoplasma gondii and Leishmania spp. in domestic cats from Luanda, Angolaen_EN
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentro de Ciência Animal e Veterinária
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FCVT%2F00772%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FCVT%2F0772%2F2016/PT
oaire.fundingStream5876
oaire.fundingStream6817 - DCRRNI ID
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccessen_EN
rcaap.typearticleen_EN
relation.isProjectOfPublicatione5b09f32-f87a-436a-95f6-17fb6f0a5a2a
relation.isProjectOfPublicationa49ceeca-4bf0-4082-971c-ece67cd1331c
relation.isProjectOfPublication.latestForDiscoverya49ceeca-4bf0-4082-971c-ece67cd1331c

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