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Photodynamic inactivation of Staphylococcus aureus by ecological antibacterial solutions associating LED (ʎ 450 ± 10 nm) with curcumin and olive leaf extracts

2023Journal article Open access
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dc.contributor.authorCrugeira, Pedro Jorge Louro
dc.contributor.authorAlmeida, Heloísa H.S.
dc.contributor.authorTeixeira, Liandra Gracher
dc.contributor.authorBarreiro, M.F.
dc.date.accessioned2022-07-14T09:57:37Z
dc.date.available2022-07-14T09:57:37Z
dc.date.issued2023
dc.description.abstractAntimicrobial resistance is a problem in contemporary society, with Staphylococcus aureus standing out as a threat due to its ability to colonize, its pathogenicity, and its expression of several virulence factors. In this context, antimicrobial photodynamic inactivation (aPDI) emerges as an alternative to conventional microbicidal or microbiostatic systems, enabling numerous and successive applications without developing side effects and microbial resistance. In this context, an aPDI system against cultures of S. aureus based on a water-in-oil (W/O) emulsion incorporating curcumin as the photosensitizer (PS), with and without olive leaf extract (OLE), was developed and the antibacterial efficacy evaluated under LED activation (lambda 450 +/- 10 nm) by depositing an energy density of 14 J/cm(2). The produced emulsified systems showed no significant differences in the droplet size and morphology, remaining stable along the tested period of 30 days. The bacterial reduction achieved after the first aPDI application for the emulsions added with curcumin and curcumin combined with the OLE was 5 log10 CFU. mL(-1) and 6 log10 CFU.mL(-1), respectively, revealing a significant difference between the two groups (p < 0.0001). After the second aPDI application, an increased microbial reduction (7 log10 CFU.mL(-1)) was observed for both studied groups even with a low significant difference (p < 0.05). The PS loading through an emulsified system for aPDI obtained a bactericidal action against S. aureus, increased by applying two aPDI, showing a significant synergy between photodynamic inactivation, OLE delivery and antibacterial activity. In addition, the developed solutions were produced using natural products by an ecologically correct process.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCrugeira, Pedro Jorge Louro; Almeida, Heloísa H.S.; Teixeira, Liandra Gracher; Barreiro, M.F.. (2023). Photodynamic inactivation of Staphylococcus aureus by ecological antibacterial solutions associating LED (ʎ 450 ± 10 nm) with curcumin and olive leaf extracts. Journal Of Photochemistry And Photobiology B-Biology. EISSN 1873-2682. 238, p. 1-7pt_PT
dc.identifier.doi10.1016/j.jphotobiol.2022.112626
dc.identifier.eissn1873-2682
dc.identifier.issn1011-1344
dc.identifier.urihttp://hdl.handle.net/10198/25644
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAntimicrobial activitypt_PT
dc.subjectW/O emulsions
dc.subjectLED
dc.subjectCurcumin
dc.subjectOlive leaf extract
dc.subjectEco-friendly antibacterial solutions
dc.titlePhotodynamic inactivation of Staphylococcus aureus by ecological antibacterial solutions associating LED (ʎ 450 ± 10 nm) with curcumin and olive leaf extractspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleJournal Of Photochemistry And Photobiology B-Biologypt_PT
person.familyNameCrugeira
person.familyNameAlmeida
person.familyNameTeixeira
person.familyNameBarreiro
person.givenNamePedro
person.givenNameHeloísa H.S.
person.givenNameLiandra Gracher
person.givenNameFilomena
person.identifier.ciencia-id8F17-E60C-A45D
person.identifier.ciencia-id8012-E230-D0DE
person.identifier.ciencia-id3910-3CB3-F50C
person.identifier.ciencia-id3418-47D5-5746
person.identifier.orcid0000-0002-7084-3711
person.identifier.orcid0000-0003-0807-3522
person.identifier.orcid0000-0003-1385-2037
person.identifier.orcid0000-0002-6844-333X
person.identifier.ridL-9802-2014
person.identifier.scopus-author-id57188719717
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscoverybf1e9976-7e16-46fc-b002-b3d31123dff6

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