Repository logo
 
Publication

Characterization of a squaraine/chitosan system for photodynamic therapy of cancer

2016Journal article Restricted access
Simple item page
dc.contributor.authorFerreira, Diana P.
dc.contributor.authorConceição, David S.
dc.contributor.authorFernandes, Fátima
dc.contributor.authorSousa, T.
dc.contributor.authorCalhelha, Ricardo C.
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorSantos, Paulo F.
dc.contributor.authorFerreira, L.F. Vieira
dc.date.accessioned2017-01-09T16:38:01Z
dc.date.available2017-01-09T16:38:01Z
dc.date.issued2016
dc.description.abstractIn this work, a squaraine dye CS5 was characterized and evaluated for its potential in photodynamic therapy. The studies were performed in ethanol and also in a powdered biopolymer, in this case chitosan. Ground state absorption, absolute fluorescence quantum yields, fluorescence lifetimes, and transient absorption were determined in order to evaluate the advantage of adsorbing the dye onto a biopolymer. Several concentrations of the dye, adsorbed onto chitosan, were prepared in order to evaluate the concentration effect on the photophysical parameters under study. A remarkable increase in the fluorescence quantum yield and lifetimes was detected when compared with the dye in solution. Also, a very clear dependence of the fluorescence quantum yield on the concentration range was found. A lifetime distribution analysis of these systems fluorescence evidenced the entrapment of the dye onto the chitosan environment with a monoexponential decay which corresponds to the monomer emission in slightly different environments. The transient absorption spectrum was obtained without sensitization indicating the existence of a triplet state which takes special importance in the generation of phototoxic species namely singlet oxygen. The subcellular localization of a photosensitizer is critical for efficient photoinduced cell death, in this way, colocalization studies were performed within HeLa cell line (human cervical carcinoma) through confocal microscopy. Toxicity in the dark and phototoxicity of CS5 were also evaluated for the same cellular model.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFerreira, Diana P.: Conceição, David S.; Fernandes, F.; Sousa, T.; Calhelha, Ricardo C.; Ferreira, Isabel C.F.R.; Santos, Paulo F.; Ferreira, L.F. Vieira (2016). Characterization of a squaraine/chitosan system for photodynamic therapy of cancer. Journal of Physical Chemistry B. ISSN 1520-6106. 120:7, p. 1212-1220pt_PT
dc.identifier.doi10.1021/acs.jpcb.5b11604pt_PT
dc.identifier.issn1520-6106
dc.identifier.urihttp://hdl.handle.net/10198/13704
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Chemical Societypt_PT
dc.relationSFRH/BPD/68334/2010pt_PT
dc.relationPHOTOSENSITIZER/BIOMATERIAL LOCAL DRUG DELIVERY SYSTEMS WITH POTENTIAL APPLICATION IN PHOTODYNAMIC THERAPY OF CANCER
dc.relationMECHANISM OF INHIBITION OF BAX-MEDIATED APOPTOSIS BY CYTOPROTECTIVE BILE ACIDS
dc.relationMODIFIED TIO2 MESOPOROUS MATERIALS AND NANOSTRUCTURES FOR PHOTOVOLTAIC CELLS WITH ENHANCED PHOTOELECTRICAL PROPERTIES
dc.relationINVESTIGATING THE RELATIONSHIP BETWEEN PI4,5P2 COMPARTMENTALIZATION AND PALMITOYLATED SYNAPTIC PROTEINS, IN THE PLASMA MEMBRANE OF NEURON MODEL CELLS
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleCharacterization of a squaraine/chitosan system for photodynamic therapy of cancerpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlePHOTOSENSITIZER/BIOMATERIAL LOCAL DRUG DELIVERY SYSTEMS WITH POTENTIAL APPLICATION IN PHOTODYNAMIC THERAPY OF CANCER
oaire.awardTitleMECHANISM OF INHIBITION OF BAX-MEDIATED APOPTOSIS BY CYTOPROTECTIVE BILE ACIDS
oaire.awardTitleMODIFIED TIO2 MESOPOROUS MATERIALS AND NANOSTRUCTURES FOR PHOTOVOLTAIC CELLS WITH ENHANCED PHOTOELECTRICAL PROPERTIES
oaire.awardTitleINVESTIGATING THE RELATIONSHIP BETWEEN PI4,5P2 COMPARTMENTALIZATION AND PALMITOYLATED SYNAPTIC PROTEINS, IN THE PLASMA MEMBRANE OF NEURON MODEL CELLS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/RECI%2FCTM-POL%2F0342%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F95359%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F92398%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F95358%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F64320%2F2009/PT
oaire.citation.endPage1220pt_PT
oaire.citation.issue7pt_PT
oaire.citation.startPage1212pt_PT
oaire.citation.titleThe Journal of Physical Chemistry Bpt_PT
oaire.citation.volume120pt_PT
oaire.fundingStream3599-PPCDT
person.familyNameCalhelha
person.familyNameFerreira
person.givenNameRicardo C.
person.givenNameIsabel C.F.R.
person.identifier144781
person.identifier.ciencia-idF313-E3CE-554E
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0002-6801-4578
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridJ-2172-2014
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id6507978333
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctThanks are due to FCT (Portugal’s Foundation for Science and Technology) for the support by scientific projects (RECI/CTM-POL/0342/2012). FCT also provided Ph.D. fellowships for D.P.F. (SFRH/BD/95359/2013), T.S. (SFRH/BD/92398/2013), and D.C. (SFRH/BD/95358/2013), as well as postdoctoral fellowships to F.F. (SFRH/BPD/64320/2009) and R.C.C. (SFRH/BPD/68334/2010).pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication2d5d1a41-7561-4a01-871c-b4c97da35053
relation.isAuthorOfPublicationbd0d1537-2e03-41fb-b27a-140af9c35db8
relation.isAuthorOfPublication.latestForDiscoverybd0d1537-2e03-41fb-b27a-140af9c35db8
relation.isProjectOfPublicationcb387cb8-8ba9-45ec-8a20-a6c7b56852c5
relation.isProjectOfPublication9e965be1-53c4-403a-9e50-f189b6b84312
relation.isProjectOfPublication9595658d-2dec-4c2a-9d3c-214689f30330
relation.isProjectOfPublicationacfe44c8-1618-472a-a724-7104ba6b7964
relation.isProjectOfPublication8a23f591-de23-467f-b014-baf120f36192
relation.isProjectOfPublication.latestForDiscovery9e965be1-53c4-403a-9e50-f189b6b84312

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Artigo 363.pdf
Size:
3.66 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CIMO - Artigos em Revistas Indexados à WoS/Scopus