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Advisor(s)
Abstract(s)
Chestnut blight, caused by Cryphonectria parasitica, is a severe disease that may be biologically
controlled by the use of hypovirulent strains, but the diversity of the pathogen population
affects biocontrol feasibility. Villuercas–Ibores–Jara, the Jerte Valley and La Vera are the main chestnut
production districts in Cáceres (central-western Spain). The purpose of this study is to determine
the Vegetative Compatibility Groups (VCGs) and mating types in these districts and to identify
hypovirulent isolates to be used in biological control. The VCGs were determined by the merging/
barrage response; PCR tests were used for the mating type determination and CHV-1 hypovirus
detection. In total, 40 sites were surveyed and 269 isolates were obtained, most of them (227) from the
Villuercas–Ibores–Jara district, where EU11 was the predominant VCG (88.1%) with EU1 (6.6%) and
EU12 (4.4%) being also present. In the Jerte Valley and La Vera, EU1 (61.9%), EU11 (11.9%) and EU12
(11.9%) were the predominant VCGs. Both mating types were detected (48% MAT-1; 50% MAT-2) and
in general, only one mating type was found in each site. The presence of Cryphonectria hypovirus 1
(CHV-1), subtype I, was identified in only one isolate (VCG EU11) from Villuercas–Ibores–Jara. The
characteristics of the C. parasitica population in this district and the occurrence of CHV-1 hypovirus
support the potential of successful biological control in Villuercas–Ibores–Jara using hypovirulent
strains, while in the Jerte Valley and La Vera only preventive measures are recommended.
Description
Keywords
Cryphonectria hypovirus Hypovirulence Mating types Preventive measures Vegetative compatibility groups
Citation
Rodríguez-Molina, María del Carmen; García-García, María Blanca; Osuna, María Dolores; Gouveia, Eugénia; Serrano-Pérez, Paula (2023). Various population structures of Cryphonectria parasitica in Cáceres (Spain) determine the feasibility of the biological control of chestnut blight with hypovirulent strains. Agronomy. eISSN 2073-4395. 13:5, p. 1-13
Publisher
MDPI