Logo do repositório
 
Publicação

Widespread benzimidazole resistance and β-tub-1 F200Y SNP in gastrointestinal nematodes of sheep across Portugal

2026Artigo científico Acesso aberto
Ver registo simples
datacite.subject.fosCiências Agrárias::Ciências Veterinárias
datacite.subject.fosCiências Naturais::Ciências Biológicas
datacite.subject.sdg04:Educação de Qualidade
datacite.subject.sdg03:Saúde de Qualidade
datacite.subject.sdg15:Proteger a Vida Terrestre
dc.contributor.authorCampbell, Natália Faria
dc.contributor.authorAlmeida, Paulo
dc.contributor.authorSimões, Ana Rita
dc.contributor.authorAntunes, Joel
dc.contributor.authorQuintas, Hélder
dc.contributor.authorPires, Humberto
dc.contributor.authorMonteiro, Helena
dc.contributor.authorWaap, Helga
dc.contributor.authorCortes, Helder
dc.date.accessioned2026-06-24T16:07:49Z
dc.date.available2026-06-24T16:07:49Z
dc.date.issued2026
dc.description.abstractAnthelmintic resistance (AR) to benzimidazoles (BZs) in gastrointestinal nematodes (GINs) of sheep was assessed across 35 farms in northern (Trás-os-Montes, TM), north-central (Beira Interior, BI), central (Estremadura, ES), and southern (Alentejo, AL) Portugal. Pooled fecal samples were tested for AR by egg hatch tests (EHTs) with albendazole (ABZ) and thiabendazole (TBZ). Effective concentrations inhibiting 50% egg hatch (EC50) were calculated, with EC50 > 0.13 µg/mL (ABZ) and > 0.10 µg/mL (TBZ) indicating AR. ABZ resistance was detected on 69% of farms, while TBZ, never administered, showed resistance on 59% of farms, suggesting wide BZ cross-resistance. EC50 values ranged from 0.05 to 0.68 µg/mL (ABZ) and 0.03–0.70 µg/mL (TBZ). Nemabiome metabarcoding identified 10 GIN species, predominantly Haemonchus contortus (41%), Teladorsagia circumcincta (33%), and Trichostrongylus colubriformis (19%). Less common species included Bunostomum trigonocephalum and Oesophagostomum venulosum. Species richness (3–6 per farm) was highest in TM, with unique occurrences of B. trigonocephalum and Cooperia sp. H. contortus dominated in TM and BI, while T. circumcincta prevailed in AL, where AR was most severe. Higher EC50 values correlated with increased T. circumcincta and T. colubriformis prevalence. The BZ-resistance F200Y SNP in the isotype-1 β-tubulin gene of H. contortus was detected on 85% of farms, with resistant allele frequencies inferring predominantly homozygous resistant populations (A/A; > 90% resistant allele) on some farms, strongly correlating with higher EC50 values, particularly in AL. Widespread BZ resistance underscores the need for enhanced AR surveillance, prudent anthelmintic use, and integrated management to sustain sheep health in Portugal, especially in Alentejo.eng
dc.description.sponsorshipThis study was chiefly financed privately by N Faria Campbell and funded by National Funds through the FCT – Foundation for Science and Technology under Project UID/05183/2025. Some supplies and use of research facilities was provided by Instituto Nacional de Investigação Agrária e Veterinária, I.P. (INIAV) and Associate Laboratory CHANGE – Global Change and Sustainability Institute (https://doi.org/10.54499/LA/P/0121/2020).
dc.identifier.citationCampbell, Natália Faria; Almeida, Paulo; Simões, Ana Rita; Antunes, Joel; Quintas, Hélder; Pires, Humberto; Monteiro, Helena; Waap, Helga; Cortes, Helder (2026). Veterinary Parasitology. Widespread benzimidazole resistance and β-tub-1 F200Y SNP in gastrointestinal nematodes of sheep across Portugal. ISSN 0304-4017. 345, p. 1-10
dc.identifier.doi10.1016/j.vetpar.2026.110782
dc.identifier.issn0304-4017
dc.identifier.urihttp://hdl.handle.net/10198/36933
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationCHANGE - Global Change and Sustainability Institute
dc.relationInstituto Mediterrâneo para a Agricultura, Ambiente e Desenvolvimento - UID/05183/2025
dc.relation.ispartofVeterinary Parasitology
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAnthelmintic resistance
dc.subjectGastrointestinal nematodes
dc.subjectβ-tubulin
dc.subjectF200Y SNP
dc.subjectSheep
dc.titleWidespread benzimidazole resistance and β-tub-1 F200Y SNP in gastrointestinal nematodes of sheep across Portugaleng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberLA/P/0121/2020
oaire.awardNumberUID/05183/2025
oaire.awardTitleCHANGE - Global Change and Sustainability Institute
oaire.awardTitleInstituto Mediterrâneo para a Agricultura, Ambiente e Desenvolvimento - UID/05183/2025
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0121%2F2020/PT
oaire.awardURIhttps://sciproj.ptcris.pt/177080UID/
oaire.citation.endPage10
oaire.citation.startPage1
oaire.citation.titleVeterinary Parasitology
oaire.citation.volume345
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamAvaliação UID 2023/2024
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameQuintas
person.givenNameHélder
person.identifier1486884
person.identifier.ciencia-id6018-8BBD-80AB
person.identifier.orcid0000-0002-6934-1669
person.identifier.scopus-author-id35796342900
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isAuthorOfPublication068e74bc-656d-4fd1-93ed-f7346baa3b69
relation.isAuthorOfPublication.latestForDiscovery068e74bc-656d-4fd1-93ed-f7346baa3b69
relation.isProjectOfPublication5a890098-6ee4-4463-8f30-ad1f52f0072b
relation.isProjectOfPublication67505f71-091c-4f3d-8744-8cd5ec444fce
relation.isProjectOfPublication.latestForDiscovery5a890098-6ee4-4463-8f30-ad1f52f0072b

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
Widespread benzimidazole.pdf
Tamanho:
1.98 MB
Formato:
Adobe Portable Document Format
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
1.75 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

CIMO - Artigos em Revistas Indexados à WoS/Scopus