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Exploring the Potential of Hop (Humulus lupulus) Cone Residue: Chemical Characterization and Evaluation of Bioactivities

2026Artigo científico Acesso aberto
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datacite.subject.fosCiências Agrárias::Biotecnologia Agrária e Alimentar
datacite.subject.fosCiências Naturais::Ciências Químicas
datacite.subject.sdg12:Produção e Consumo Sustentáveis
datacite.subject.sdg09:Indústria, Inovação e Infraestruturas
datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorReyes, Giulia Boito
dc.contributor.authorSantos, Emylaine Pereira dos
dc.contributor.authorSantos, Everton da Silva
dc.contributor.authorGonçalves, Laura Correia
dc.contributor.authorSilva, Gabriela Catuzo Canonico
dc.contributor.authorGazim, Zilda Cristiani
dc.contributor.authorGonçalves, Regina Aparecida Correia
dc.contributor.authorOliveira, Arildo José Braz de
dc.contributor.authorPinela, José
dc.contributor.authorMandim, Filipa
dc.contributor.authorPires, Tânia C.S.P.
dc.contributor.authorCardozo-Filho, Lucio
dc.contributor.authorCorrêa, Rúbia C.G.
dc.contributor.authorGonçalves, José Eduardo
dc.date.accessioned2026-07-02T10:35:24Z
dc.date.available2026-07-02T10:35:24Z
dc.date.issued2026
dc.description.abstractHumulus lupulus L. (hops), belonging to the Cannabaceae family, is grown mainly for brewing, with 98% of global production directed to this sector. Moreover, large volumes of female cone residues are generated as by-products, representing a valuable source of bioactive compounds that can be valorized under green chemistry principles. This study aimed to extract bioactive compounds from hop cone residues sourced from craft breweries using ultrasound-assisted (EH-UA) and microwave-assisted (EH-MA) extraction methods. Hydroalcoholic extracts (70%) were analyzed for chemical composition, antioxidant, antimicrobial, antiproliferative, nitric oxide (NO)-production inhibition, and photoprotective activities. GC-MS identified 32 compounds in EH-MA and 30 in EH-UA, including terpenes, sesquiterpenes, oxygenated sesquiterpenes, and fatty acids. Both extracts demonstrated strong antioxidant activity in cell-based (TBARS, OxHLIA) and chemical (DPPH, ABTS, FRAP) assays, particularly EH-MA. Significant antibacterial activity was observed, especially against Enterobacter cloacae, Pseudomonas aeruginosa, and Staphylococcus aureus (MIC 1–10 mg/mL), as well as antifungal activity against Aspergillus brasiliensis (MIC 2–2.5 mg/mL). Selective antiproliferative activity was observed against tumor cell lines Caco-2 and MCF-7 (GI50 25 μg/mL), without cytotoxicity toward nontumor cell lines Vero and PLP2 (GI50 > 400 μg/mL). All extracts inhibited the production of the inflammation mediator NO, with EH-MA showing the most potent effect (IC50 of 35 μg/mL), followed by EH-UA (IC50 of 55 μg/mL). Photoprotective potential was also demonstrated, with SPF values of 19 (EH-MA) and 18 (EH-UA). In conclusion, hop cone residues can yield multifunctional extracts with antioxidant, antimicrobial, antiproliferative, anti-inflammatory, and photoprotective activities, which support their sustainable upcycling for pharmacological, nutraceutical, and cosmetic applications.eng
dc.description.sponsorshipThe authors extend gratitude to the CNPq (309838/2022-3, 308505/2019-2, and 313009/2025-2), the CAPES, and research contributions from ICETI, Unicesumar, IPB, and UEM. This work was also supported by FCT/MCTES (PIDDAC): CIMO, UIDB/00690/2020, UIDP/00690/2020, and SusTEC, LA/P/0007/2020.
dc.identifier.citationReyes, Giulia Boito; Santos, Emylaine Pereira dos; Santos, Everton da Silva; Gonçalves, Laura Correia; Silva, Gabriela Catuzo Canonico; Gazim, Zilda Cristiani; Gonçalves, Regina Aparecida De Correia; Oliveira, Arildo Jose Braz; Pinela, Jose; Mandim, Filipa; Pire, Tania C. S. P.; Cardozo-Filho, Lucio; Correa, Rubia Carvalho Gomes; Gonçalves, Jose Eduardo (2026). Exploring the Potential of Hop (Humulus lupulus) Cone Residue: Chemical Characterization and Evaluation of Bioactivities. Plants. ISSN 2223-7747. 15:7, p. 1-16
dc.identifier.doi10.3390/plants15070994
dc.identifier.issn2223-7747
dc.identifier.urihttp://hdl.handle.net/10198/36969
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationMountain Research Center - UIDP/00690/2020
dc.relationMountain Research Center - UIDB/00690/2020
dc.relationAssociate Laboratory for Sustainability and Tecnology in Mountain Regions - LA/P/0007/2020
dc.relation.ispartofPlants
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectAntioxidant bioactivity
dc.subjectGreen chemistry
dc.subjectHhop cone residues
dc.subjectMicrowave and ultrasound assisted extraction
dc.subjectPharmacological potential
dc.titleExploring the Potential of Hop (Humulus lupulus) Cone Residue: Chemical Characterization and Evaluation of Bioactivitieseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberUIDP/00690/2020
oaire.awardNumberUIDB/00690/2020
oaire.awardNumberLA/P/0007/2020
oaire.awardTitleMountain Research Center - UIDP/00690/2020
oaire.awardTitleMountain Research Center - UIDB/00690/2020
oaire.awardTitleAssociate Laboratory for Sustainability and Tecnology in Mountain Regions - LA/P/0007/2020
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0007%2F2020/PT
oaire.citation.endPage16
oaire.citation.issue7
oaire.citation.startPage1
oaire.citation.titlePlants
oaire.citation.volume15
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNamePinela
person.familyNameMandim
person.familyNamePires
person.familyNameCorrêa
person.givenNameJosé
person.givenNameFilipa
person.givenNameTânia C.S.P.
person.givenNameRúbia C.G.
person.identifier2118829
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person.identifier.ciencia-id321E-2D96-00CA
person.identifier.ciencia-id6C1F-2818-CBB5
person.identifier.orcid0000-0001-7523-1637
person.identifier.orcid0000-0002-6494-2326
person.identifier.orcid0000-0002-3954-3833
person.identifier.orcid0000-0003-0269-4344
person.identifier.ridB-4466-2014
person.identifier.ridQ-2842-2018
person.identifier.scopus-author-id54392272800
person.identifier.scopus-author-id57057440000
person.identifier.scopus-author-id37661286600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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