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Banana Peels as Substrate for Lactic Acid Production: Upstream Fermentation Bioprocess

2025Journal article Open access
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datacite.subject.fosCiências Agrárias::Biotecnologia Agrária e Alimentar
datacite.subject.fosCiências Naturais::Ciências Biológicas
datacite.subject.fosCiências Naturais::Ciências da Terra e do Ambiente
datacite.subject.sdg12:Produção e Consumo Sustentáveis
datacite.subject.sdg04:Educação de Qualidade
dc.contributor.authorPedrosa, Mariana C.
dc.contributor.authorHeleno, Sandrina A.
dc.contributor.authorAlvarez, Cristina
dc.contributor.authorSusmozas, Ana
dc.contributor.authorBallesteros, Ignacio
dc.contributor.authorPintado, Manuela
dc.contributor.authorBarros, Lillian
dc.contributor.authorCarocho, Márcio
dc.date.accessioned2025-11-05T19:10:56Z
dc.date.available2025-11-05T19:10:56Z
dc.date.issued2025
dc.description.abstractPurpose Banana peels account for around 30-40% of the banana's weight and represent an industrial waste. This biomass can be used as an eco-friendly solution for biotechnological bioprocesses. Still, due to the fibrous nature of banana peels, pretreatment becomes essential to enhance the effectiveness of enzymes on the hydrolysis of complex carbohydrates. This study aims to determine the best pretreatment and enzymatic hydrolysis conditions to produce a fermentation substrate suitable for producing lactic acid from the peels.MethodsA diluted H2SO4 (0.5% and 0.25%) was used in an autoclave for 10 min at 121 degrees C. The pretreated material was subjected to several enzymatic hydrolysis tests in which two variables were evaluated for their influence: solid load (10, 15 and 20%), and H2SO4 concentration (0.5 and 0.25% v/v). Commercial enzymes (cellulolytic enzyme mixtures and pectinase) were used.ResultsAll enzymatic hydrolysis yields of cellulose referring to glucose released in the hydrolysates surpassed 60% in all the conditions tested after pretreatment. After evaluating the pretreatment efficiency, the ideal parameters were defined as 0.25% H2SO4, and cellulolytic enzyme mixtures with 15 and 20% solid load. Subsequently, a screening was carried out in MRS + broth with the most suitable pH and temperature conditions for lactic acid production by Lacticaseibacillus rhamnosus.ConclusionsThe results showed the potential of BP medium for lactic acid production, with yields of 0.91 g/g sugar in sugar-containing media from enzymatic hydrolysis at 15% solid load and 0.82 g/g sugar in media from 20% solid load assays.eng
dc.description.sponsorshipThis work was supported by national funds through FCT/MCTES (PIDDAC): CIMO, UIDB/00690/2020 (DOI: https://doi.org/10.54499/UIDB/00690/2020) and UIDP/00690/2020 (DOI: https://doi.org/10.54499/UIDP/00690/2020); and SusTEC, LA/P/0007/2020 (DOI: https://doi.org/10.54499/LA/P/0007/2020). RESOPLA (Grant TED2021-132024B-C22 funded by MCIU/AEI/ https://doi.org/10.13039/501100011033 and by the “European Union NextGenerationEU/PRTR). To National funding by FCT- Foundation for Science and Technology, through the institutional scientific employment program-contract with S.A. Heleno and L. Barros (CEEC-INST, DOI: https://doi.org/10.54499/CEECINST/00107/2021/CP2793/CT0002), as well as M. Carocho for his individual contract (CEEC-IND/00831/2018), while M.C. Pedrosa’s acknowledges FCT for her PhD grant (DOI https://doi.org/10.54499/2021.04531.BD).
dc.identifier.citationPedrosa, Mariana C.; Heleno, Sandrina; Alvarez, Cristina; Susmozas, Ana; Ballesteros, Ignacio; Pintado, Manuela; Barros, Lillian; Carocho, Marcio (2025). Banana Peels as Substrate for Lactic Acid Production: Upstream Fermentation Bioprocess. Waste and Biomass Valorization. ISSN 1877-2641. p. 1-17
dc.identifier.doi10.1007/s12649-025-03323-y
dc.identifier.issn1877-2641
dc.identifier.issn1877-265X
dc.identifier.urihttp://hdl.handle.net/10198/34969
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer Nature
dc.relationMountain Research Center
dc.relationAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
dc.relation.ispartofWaste and Biomass Valorization
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectBiowaste
dc.subjectCarbon source
dc.subjectLactic acid
dc.subjectHydrolysis
dc.titleBanana Peels as Substrate for Lactic Acid Production: Upstream Fermentation Bioprocesseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardTitleAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0007%2F2020/PT
oaire.citation.endPage17
oaire.citation.startPage1
oaire.citation.titleWaste and Biomass Valorization
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNamePedrosa
person.familyNameHeleno
person.familyNameBarros
person.familyNameCarocho
person.givenNameMariana C.
person.givenNameSandrina A.
person.givenNameLillian
person.givenNameMárcio
person.identifier469085
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person.identifier.ciencia-id5614-005B-5CF1
person.identifier.orcid0000-0002-2057-0639
person.identifier.orcid0000-0001-7224-1098
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0002-8978-4547
person.identifier.ridL-5951-2014
person.identifier.ridJ-3600-2013
person.identifier.scopus-author-id30067731800
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id52463229800
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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