Publication
Quantitative detection of soybean in meat products by a TaqMan real-time PCR assay
| dc.contributor.author | Soares, Sónia | |
| dc.contributor.author | Amaral, Joana S. | |
| dc.contributor.author | Oliveira, Beatriz | |
| dc.contributor.author | Mafra, Isabel | |
| dc.date.accessioned | 2018-02-19T10:00:00Z | |
| dc.date.accessioned | 2018-03-08T15:14:47Z | |
| dc.date.available | 2018-01-19T10:00:00Z | |
| dc.date.available | 2018-03-08T15:14:47Z | |
| dc.date.issued | 2014 | |
| dc.description.abstract | In the present work, we propose a normalised real-time quantitative PCR assay to determine the addition of soybean to meat products. The method proved to be a powerful tool for the quantification of soybean protein (dry basis) in the range of 0.01% to 6%, being successfully in-house validated. Its application was effective in the analysis of several meat products, indicating 2% of non-compliance with the food allergen labelling legislation, and some inconsistencies when comparing the declared with estimated amounts of soybean. This work highlights the importance of efficient tools to assess labelling statements of meat products, avoiding fraudulent practices. | en_EN |
| dc.description.sponsorship | This work has been supported by Fundação para a Ciência e a Tecnologia (FCT) through grant no. PEst-C/EQB/LA0006/2013 and the University of Porto “Projectos Pluridisciplinares” IJUP2011-149. S. Soares is grateful to FCT PhD grant (SFRH/BD/75091/2010) financed by POPH— QREN (subsidised by FSE and MCTES). | |
| dc.description.version | info:eu-repo/semantics/publishedVersion | en_EN |
| dc.identifier.citation | Soares, Sónia; Amaral, Joana S.; Oliveira, M. Beatriz P.P.; Mafra, Isabel (2014). Quantitative detection of soybean in meat products by a TaqMan real-time PCR assay. Meat Science. ISSN 0309-1740. 98, p. 41-46 | en_EN |
| dc.identifier.doi | 10.1016/j.meatsci.2014.04.002 | en_EN |
| dc.identifier.issn | 0309-1740 | |
| dc.identifier.uri | http://hdl.handle.net/10198/16218 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | en_EN |
| dc.subject | Adulteration | en_EN |
| dc.subject | Food allergen | en_EN |
| dc.subject | Meat products | en_EN |
| dc.subject | Real-time PCR | en_EN |
| dc.subject | Soybean detection | en_EN |
| dc.title | Quantitative detection of soybean in meat products by a TaqMan real-time PCR assay | en_EN |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/COMPETE/PEst-C%2FEQB%2FLA0006%2F2013/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F75091%2F2010/PT | |
| oaire.fundingStream | COMPETE | |
| oaire.fundingStream | SFRH | |
| person.familyName | Amaral | |
| person.givenName | Joana S. | |
| person.identifier.ciencia-id | 5319-7DE8-BEDA | |
| person.identifier.orcid | 0000-0002-3648-7303 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | en_EN |
| rcaap.type | article | en_EN |
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