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Advisor(s)
Abstract(s)
In this study, four different protocols were
tested for their ability to extract DNA from blended refined
vegetable oils: the in-house prepared Wizard and CTAB
methods and the methods based on the use of the commercial
kits Wizard Magnetic DNA purification system
for food and Nucleospin for food. The performance of the
extraction protocols was determined by end-point polymerase
chain reaction (PCR) targeting the soybean lectin
gene with primers suitable for the amplification of small
fragments and confirmed by real-time PCR with specific
hydrolysis probes. From the tested protocols, the Nucleospin
method was the only one able to produce amplifiable
DNA from refined vegetable oils. To verify the presence of
Roundup Ready (RR) soybean, event-specific primers
were used for end-point PCR assays. The amplification of
trace amounts of RR soybean by real-time PCR confirmed
the label statements of two samples. The results highlight
the importance of the DNA extraction protocol and the
critical choice of PCR primers on processed food matrices,
such as refined oils. Considering the few reports and difficulties
pointed out in the literature to obtain amplifiable
DNA from refined vegetable oils, the present results can be
a step forward in the traceability of refined oils regarding
authenticity issues and genetically modified organism
detection.
Description
Keywords
Soybean oil Vegetable oil DNA extraction Real-time PCR GMO detection Refined oil
Citation
Costa, J.; Mafra, I.; Amaral, J.S.; Oliveira, M.B.P.P (2010). Detection of genetically modified soybean DNA in refined vegetable oils. European Food Research and Technology. ISSN 1438-2377. 230:6, p.915-923
Publisher
European Food Research and Technology