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HPLC method for simultaneous detection of aflatoxins and cyclopiazonic acid

dc.contributor.authorSoares, Célia
dc.contributor.authorRodrigues, Paula
dc.contributor.authorFreitas-Silva, Otniel
dc.contributor.authorAbrunhosa, Luís
dc.contributor.authorVenâncio, Armando
dc.date.accessioned2011-03-11T11:17:11Z
dc.date.available2011-03-11T11:17:11Z
dc.date.issued2010
dc.description.abstractAspergillus species in section Flavi are among the most relevant mycotoxigenic fungi. The organisms are well-known producers of the highly carcinogenic aflatoxins and of other mycotoxins, such as cyclopiazonic acid. Aflatoxins and cyclopiazonic acid analyses can be routinely used for identification purposes within the section. Two separate chromatographic runs with distinct columns and detectors for each toxin were required in previous reports. A straightforward high performance liquid chromatography (HPLC) procedure for the simultaneous detection of these compounds in fungal cultures was developed in the present work using a methanol/water mobile phase, post-column photochemical derivatisation and fluorescence detection. The proposed method was tested with standards and fungal extracts of 24 Aspergillus section Flavi strains and compared to the common individual detection of these mycotoxins by HPLC analyses.por
dc.identifier.citationSoares, C.; Rodrigues, P.; Freitas-Silva, O.; Abrunhosa, L.; Venancio, A. (2010) HPLC method for simultaneous detection of aflatoxins and cyclopiazonic acid. World Mycotoxin Journal. ISSN 1875-0710. 3:3, p. 225-231por
dc.identifier.doi10.3920/WMJ2010.1216
dc.identifier.issn1875-0710
dc.identifier.urihttp://hdl.handle.net/10198/3624
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherWageningen Academic Publisherspor
dc.relationAVALIAÇÃO DA INCIDÊNCIA DE FUNGOS FILAMENTOSOS E DE MICOTOXINAS EM MILHO NACIONAL
dc.relationCARACTERIZAÇÃO DA MICOFLORA E DO PERFIL DE MICOTOXINAS NA AMÊNDOA E CASTANHA DE TRÁS-OS-MONTES DESDE A PRODUÇÃO À COMERCIALIZAÇÃO
dc.relationDESCONTAMINAÇAO BIOLÓGICA DE OCRATOXINA A UTILIZANDO UM ENZIMA DE A. NIGER
dc.subjectAspergillus section Flavipor
dc.subjectAspergillus section Flavipor
dc.subjectFungal extractspor
dc.subjectDual mycotoxin detection
dc.titleHPLC method for simultaneous detection of aflatoxins and cyclopiazonic acidpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAVALIAÇÃO DA INCIDÊNCIA DE FUNGOS FILAMENTOSOS E DE MICOTOXINAS EM MILHO NACIONAL
oaire.awardTitleCARACTERIZAÇÃO DA MICOFLORA E DO PERFIL DE MICOTOXINAS NA AMÊNDOA E CASTANHA DE TRÁS-OS-MONTES DESDE A PRODUÇÃO À COMERCIALIZAÇÃO
oaire.awardTitleDESCONTAMINAÇAO BIOLÓGICA DE OCRATOXINA A UTILIZANDO UM ENZIMA DE A. NIGER
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F37264%2F2007/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F28332%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F43922%2F2008/PT
oaire.citation.endPage231por
oaire.citation.startPage225por
oaire.citation.titleWorld Mycotoxin Journalpor
person.familyNameRodrigues
person.givenNamePaula
person.identifier.ciencia-id2312-18AE-DA42
person.identifier.orcid0000-0002-3789-2730
person.identifier.scopus-author-id7102639646
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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relation.isAuthorOfPublication.latestForDiscoveryb864b8c1-d66a-419c-b1ac-e6193f93eca6
relation.isProjectOfPublication8a46ca75-f443-45bf-acb5-9ce0c6a53e50
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