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High efficacy of ozonated oils on the removal of biofilms produced by methicillin-resistant staphylococcus aureus (MRSA) from infected diabetic foot ulcers

2020Journal article Open access
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dc.contributor.authorSilva, Vanessa
dc.contributor.authorPeirone, Cecília
dc.contributor.authorAmaral, Joana S.
dc.contributor.authorCapita, Rosa
dc.contributor.authorAlonso-Calleja, Carlos
dc.contributor.authorMarques-Magallanes, José A.
dc.contributor.authorMartins, Ângela
dc.contributor.authorCarvalho, Águeda
dc.contributor.authorMaltez, Luís
dc.contributor.authorPereira, José Eduardo
dc.contributor.authorCapelo, José Luís
dc.contributor.authorIgrejas, Gilberto
dc.contributor.authorPoeta, Patrícia
dc.date.accessioned2018-02-19T10:00:00Z
dc.date.accessioned2021-02-27T16:30:54Z
dc.date.available2018-01-19T10:00:00Z
dc.date.available2021-02-27T16:30:54Z
dc.date.issued2020
dc.description.abstract© 2020 by the authors. Ozone has a high wound healing capacity and antibacterial properties and can be used as a complementary treatment in infections. Methicillin-resistant S. aureus (MRSA) is the most common pathogen found in infected diabetic foot ulcers. Most of MRSA are resistant to several classes of antibiotics and, therefore, there is a need for new, effective, and well-tolerated agents. Thus, we aimed evaluate the antimicrobial and antibiofilm potentials of ozonated vegetable oils against MRSA strains isolated from diabetic foot ulcers. Six ozonated oils were produced with concentrations of ozone ranging from 0.53 to 17 mg of ozone/g of oil. The peroxide values were determined for each oil. Ozonated oils content on fatty acid was determined by gas chromatography equipped with a flame ionization detector. The antimicrobial susceptibility testing was performed by the Kirby-Bauer disk diffusion method and the effect of ozonated oils on biofilm formation ability and on established biofilms was investigated. In general, the content in identified unsaturated fatty acid in oils decreased with the increase of ozonation time and, consequently, the peroxide value increased. Most bacterial strains were inhibited by ozonated oil at a concentration of 4.24 mg/g. Ozonated oils had moderate to high ability to remove adhered cells and showed a high capacity to eradicate 24 h old biofilms. Our results show promising use of ozonated oils on the treatment of infections, in particular those caused by multidrug-resistant MRSA strains.en_EN
dc.description.sponsorshipThis work was funded by the R&D Project CAREBIO2—Comparative assessment of antimicrobial resistance in environmental biofilms through proteomics—towards innovative theranostic biomarkers, with reference NORTE-01-0145-FEDER-030101 and PTDC/SAU-INF/30101/2017, financed by the European Regional Development Fund (ERDF) through the Northern Regional Operational Program (NORTE 2020) and the Foundation for Science and Technology (FCT). This work was supported by the Associate Laboratory for Green Chemistry—LAQV, which is financed by national funds from FCT/MCTES (UIDB/50006/2020). Joana S. Amaral is grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support by national funds FCT/MCTES to CIMO (UIDB/00690/2020). Vanessa Silva is grateful to FCT (Fundação para a Ciência e a Tecnologia) for financial support through PhD grant SFRH/BD/137947/2018.
dc.description.versioninfo:eu-repo/semantics/publishedVersionen_EN
dc.identifier.citationSilva, Vanessa; Peirone, Cecília; Amaral, Joana S.; Capita, Rosa; Alonso-Calleja, Carlos; Marques-Magallanes, José A.; Martins, Ângela; Carvalho, Águeda; Maltez, Luís; Pereira, José Eduardo; Capelo, José Luís; Igrejas, Gilberto; Poeta, Patrícia (2020). High efficacy of ozonated oils on the removal of biofilms produced by methicillin-resistant staphylococcus aureus (MRSA) from infected diabetic foot ulcers. Molecules. ISSN 1420-3049. 25:16, p. 1-13en_EN
dc.identifier.doi10.3390/molecules25163601en_EN
dc.identifier.urihttp://hdl.handle.net/10198/23399
dc.language.isoeng
dc.peerreviewedyesen_EN
dc.relationRTI2018-098267-R-C33
dc.subjectBiofilmsen_EN
dc.subjectDiabetic foot ulcersen_EN
dc.subjectMRSAen_EN
dc.subjectOzonated oilen_EN
dc.subjectOzoneen_EN
dc.subjectS. aureusen_EN
dc.titleHigh efficacy of ozonated oils on the removal of biofilms produced by methicillin-resistant staphylococcus aureus (MRSA) from infected diabetic foot ulcersen_EN
dc.typejournal article
dspace.entity.typePublication
person.familyNameAmaral
person.givenNameJoana S.
person.identifier.ciencia-id5319-7DE8-BEDA
person.identifier.orcid0000-0002-3648-7303
rcaap.rightsopenAccessen_EN
rcaap.typearticleen_EN
relation.isAuthorOfPublication42be2cf4-adc4-4e7f-ac60-7aab515b38cd
relation.isAuthorOfPublication.latestForDiscovery42be2cf4-adc4-4e7f-ac60-7aab515b38cd

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