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Evaluation of berberine nanoparticles as a strategy to modulate acetylcholinesterase activity

2023Journal article Open access
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dc.contributor.authorLeimann, Fernanda Vitória
dc.contributor.authorSouza, Luma Borges de
dc.contributor.authorOliveira, Byanca Pereira Moreira de
dc.contributor.authorRossi, Bruna Franzon
dc.contributor.authorSilva, Patricia Sabino da
dc.contributor.authorShiraishi, Carlos Seiti Hurtado
dc.contributor.authorKaplum, Vanessa
dc.contributor.authorAbreu, Rui M.V.
dc.contributor.authorPereira, Carla
dc.contributor.authorBarros, Lillian
dc.contributor.authorPeron, Ana Paula
dc.contributor.authorIneu, Rafael Porto
dc.contributor.authorOechsler, Bruno Francisco
dc.contributor.authorSayer, Claudia
dc.contributor.authorAraujo, Pedro Henrique Hermes de
dc.contributor.authorGonçalves, Odinei Hess
dc.date.accessioned2013-01-09T11:12:46Z
dc.date.available2013-01-09T11:12:46Z
dc.date.issued2023
dc.description.abstractResearchers have concentrated efforts in the search for natural-based reversible inhibitors for cholinesterase enzymes as they may play a key role in the treatment of degenerative diseases. Diverse plant alkaloids can inhibit the action of acetylcholinesterase and, among them, berberine is a promising bioactive. However, berberine has poor water solubility and low bioavailability, which makes it difficult to use in treatment. The solid dispersion technique can improve the water affinity of hydrophobic substances, but berberine solid dispersions have not been extensively studied. Safety testing is also essential to ensure that the berberine-loaded solid dispersions are safe for use. This study investigated the effectiveness of berberine-loaded solid dispersions (SD) as inhibitors of acetylcholinesterase enzyme (AChE). Docking simulation was used to investigate the influence of berberine on AChE, and in vitro assays were conducted to confirm the enzymatic kinetics of AChE in the presence of berberine. Berberine SD also showed improved cytotoxic effects on tumoral cells when dispersed in aqueous media. In vivo assays using Allium cepa were implemented, and no cytotoxicity/genotoxicity was found for the berberine solid dispersion. These results suggest that berberine SD could be a significant step towards safe nanostructures for use in the treatment of neurodegenerative diseases.por
dc.description.sponsorshipThe authors are grateful to CNPq and CAPES (Finance Code 001) for the support. The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES to CIMO (UIDB/00690/2020). The authors are also grateful to FCT for financial support through national funds FCT/MCTES (PIDDAC) to CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2020). L. Barros and C. Pereira thank FCT, P.I., for the contracts through the institutional scientific employment programcontract.
dc.identifier.citationLeimann, Fernanda Vitória; Souza, Luma Borges de; Oliveira, Byanca Pereira Moreira de; Rossi, Bruna Franzon; Silva, Patricia Sabino da; Shiraishi, Carlos Seiti Hurtado; Kaplum, Vanessa; Abreu, Rui M.V.; Pereira, Carla; Barros, Lillian; Peron, Ana Paula; Ineu, Rafael Porto; Oechsler, Bruno Francisco; Sayer, Claudia: Araujo, Pedro Henrique Hermes de: Gonçalves, Odinei Hess (2023). Evaluation of berberine nanoparticles as a strategy to modulate acetylcholinesterase activity. Food Research International. eISSN 1873-7145. 173, p. 1-14por
dc.identifier.doi10.1016/j.foodres.2023.113295
dc.identifier.eissn1873-7145
dc.identifier.issn0963-9969
dc.identifier.urihttp://hdl.handle.net/10198/7873
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectSolid dispersionpor
dc.subjectEncapsulationpor
dc.subjectAChEpor
dc.subjectKinetic
dc.subjectBioactive
dc.subjectBerberis vulgaris
dc.titleEvaluation of berberine nanoparticles as a strategy to modulate acetylcholinesterase activitypor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleFood Research Internationalpor
person.familyNameLeimann
person.familyNameAbreu
person.familyNamePereira
person.familyNameBarros
person.familyNameGonçalves
person.givenNameFernanda Vitória
person.givenNameRui M.V.
person.givenNameCarla
person.givenNameLillian
person.givenNameOdinei Hess
person.identifier1415151
person.identifier469085
person.identifier.ciencia-id8F10-A04D-FC0D
person.identifier.ciencia-id0F19-0DE2-12A2
person.identifier.ciencia-idEF10-2739-2B70
person.identifier.ciencia-id9616-35CB-D001
person.identifier.orcid0000-0001-6230-9597
person.identifier.orcid0000-0002-7745-8015
person.identifier.orcid0000-0003-0093-771X
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0002-9528-8187
person.identifier.ridK-1629-2016
person.identifier.ridJ-3600-2013
person.identifier.scopus-author-id7003290613
person.identifier.scopus-author-id55627876424
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id9335958400
rcaap.rightsopenAccesspor
rcaap.typearticlepor
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