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Soil DNA pyrosequencing and fruitbody surveys reveal contrasting diversity for various fungal ecological guilds in chestnut orchards

2015Journal article Open access
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dc.contributor.authorBaptista, Paula
dc.contributor.authorReis, Francisca
dc.contributor.authorPereira, Eric Carvalho
dc.contributor.authorTavares, Rui Manuel
dc.contributor.authorSantos, Pedro M.
dc.contributor.authorRichard, Franck
dc.contributor.authorSelosse, Marc André
dc.contributor.authorLino-Neto, Teresa
dc.date.accessioned2018-02-19T10:00:00Z
dc.date.accessioned2018-03-01T16:04:45Z
dc.date.accessioned2018-03-05T09:19:00Z
dc.date.available2018-01-19T10:00:00Z
dc.date.available2018-03-01T16:04:45Z
dc.date.available2018-03-05T09:19:00Z
dc.date.issued2015
dc.description.abstractFungal diversity in Mediterranean forest soils is poorly documented, particularly when considering saprobic and pathogenic organisms. Next-generation sequencing (NGS) methods applied to soil fungi provide the opportunity to unveil the most inconspicuous functional guilds (e.g. saprobes) and life forms (e.g. Corticiaceae) of this tremendous diversity. We used fruitbody surveys over 2 years and soil 454 metabarcoding in Castanea sativa orchards to evaluate res pectively the reproductive (fruitbodies) and vegetative (mycelia) parts of fungal communities in three 100-year-old stands. Analysis of 839 fruitbodies and 210291 ITS1 reads revealed high fungal diversity, mainly shown by belowground analysis, with high (dominant) abundance of mycorrhizal fruitbodies and reads. Both methods displayed contrasted composition and structure of fungal communities, with Basidio- and Ascomycetes dominating above- and belowground, respectively. For the two dominant fungal guilds (i.e. ectomycorrhizal and saprobic), diversity above- and belowground overlapped weakly. This study is the first assessment of the complementarity of fruitbody surveys and NGS for analysing fungal diversity in Mediterranean ecosystems and shows that belowground methods still need to be completed by fruiting diversity to provide a comprehensive overview of the different fungal guilds. The results shed light on chestnut soil biodiversity and question the spatial distribution and synergies among fungal guilds.en_EN
dc.description.sponsorshipThis work was funded by FEDER through the Operational Competitiveness Program (COMPETE) and by Portuguese national funds through the Foundation for Science and Technology (FCT) within the scope of project PTDC/AGR-AAM/ 099556/2008 ‘Effect of the fungus Hypholoma fasciculare on the sustainability of Castanea sativa orchards’. The authors would like to thank to Biocant (especially Dr. Conceição Egas) for pyrosequencing support.
dc.description.versioninfo:eu-repo/semantics/publishedVersionen_EN
dc.identifier.citationBaptista, Paula; Reis, Francisca; Pereira, Eric; Tavares, Rui M.; Santos, Pedro M.; Richard, Franck; Selosse, Marc André; Lino-Neto, Teresa (2015). Soil DNA pyrosequencing and fruitbody surveys reveal contrasting diversity for various fungal ecological guilds in chestnut orchards. Environmental Microbiology Reports. ISSN 1758-2229. 7, p. 946-954en_EN
dc.identifier.doi10.1111/1758-2229.12336en_EN
dc.identifier.issn1758-2229
dc.identifier.urihttp://hdl.handle.net/10198/16101
dc.language.isoeng
dc.peerreviewedyesen_EN
dc.titleSoil DNA pyrosequencing and fruitbody surveys reveal contrasting diversity for various fungal ecological guilds in chestnut orchardsen_EN
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FAGR-AAM%2F099556%2F2008/PT
oaire.fundingStream5876-PPCDTI
person.familyNameBaptista
person.givenNamePaula
person.identifier.ciencia-id7D11-FE1E-CD0F
person.identifier.orcid0000-0001-6331-3731
person.identifier.scopus-author-id14051688000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccessen_EN
rcaap.typearticleen_EN
relation.isAuthorOfPublication3f35226a-b17a-4f7d-8da1-3297105cbfe9
relation.isAuthorOfPublication.latestForDiscovery3f35226a-b17a-4f7d-8da1-3297105cbfe9
relation.isProjectOfPublication67dd9919-6192-4c28-a9bc-009afc09309f
relation.isProjectOfPublication.latestForDiscovery67dd9919-6192-4c28-a9bc-009afc09309f

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