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Genetic variation of detoxification genes: from genes to proteins

2025Documento de conferência Acesso aberto
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datacite.subject.fosCiências Naturais::Ciências da Terra e do Ambiente
datacite.subject.fosCiências Agrárias::Biotecnologia Agrária e Alimentar
datacite.subject.sdg04:Educação de Qualidade
datacite.subject.sdg13:Ação Climática
datacite.subject.sdg15:Proteger a Vida Terrestre
dc.contributor.authorHenriques, Dora
dc.contributor.authorLi, Fernanda
dc.contributor.authorBashir, Sana
dc.contributor.authorQuaresma, Andreia
dc.contributor.authorLopes, Ana Rita
dc.contributor.authorTaliadoros, Demetris
dc.contributor.authorWebster, Matthew
dc.contributor.authorShiraishi, Carlos S.H.
dc.contributor.authorYadró Garcia, Carlos A.
dc.contributor.authorAbreu, Rui M.V.
dc.contributor.authorRufino, José
dc.contributor.authorRosa-Fontana, Annelise
dc.contributor.authorVerbinnen, Gilles
dc.contributor.authorGraaf, Dirk C. de
dc.contributor.authorDe Smet, Lina
dc.contributor.authorPinto, M. Alice
dc.date.accessioned2026-02-11T16:42:42Z
dc.date.available2026-02-11T16:42:42Z
dc.date.issued2025
dc.description.abstractHoney bees (Apis mellifera) are exposed to natural and synthetic xenobiotics, requiring genetic adaptations for survival. Several gene families have been implicated in insect pesticide resistance, including cytochrome P450s, glutathione-S-transferases (GSTs), esterases, and uridine diphosphate (UDP)-glycosyltransferases. This study investigates genetic variation in these detoxification gene families and predicts the structural and functional effects of non-synonymous SNPs (single nucleotide polymorphisms) on protein structure and function. We analyzed SNPs mapped to these detoxification genes extracted from over 1,500 whole genomes representing 15 subspecies and the four main honey bee lineages: M, C, A, and O. Functional annotation and variant effects were predicted using SnpEff. Allele frequencies and each SNP’s fixation index (FST) were calculated per population and evolutionary lineage. Bioinformatics and molecular modeling techniques were employed to evaluate non-synonymous SNPs’ structural and functional consequences. Protein structures were generated from FASTA files using AlphaFold3, converted from mmCIF to PDB format, and visualized in PyMOL. Functional site predictions were performed using Proteins Plus, and molecular dynamics simulations were conducted in YASARA to assess stability and conformational changes in proteins. Our results indicate many non-synonymous SNPs in some subspecies, such as A. m. jemenitica and A. m. intermissa. The genes with the highest number of non-synonymous mutations belong to the CYP family, particularly Probable cytochrome P450 6a14 and CYP9Q1. Conversely, genes such as Cytochrome P450 6k1 and Methyl farnesoate epoxidase exhibit no non-synonymous SNPs. By understanding intraspecific genetic variation, we move closer to reliably predicting how honey bee populations will respond to pesticide exposure.eng
dc.identifier.citationHenriques, Dora; Li, Fernanda; Bashir, Sana; Quaresma, Andreia; Lopes, Ana; Taliadoros, Demetris; Webster, Matthew; Shiraishi, Carlos S.H.; Yadró García, Carlos A.; Abreu, Rui M.V.; Rufino, José; Rosa-Fontana, Annelise; Verbinnen, Gilles; Graaf, Dirk de; De Smet, Lina; Pinto, M. Alice (2025). Genetic variation of detoxification genes: from genes to proteins. Apimondia 2025. p. 64
dc.identifier.urihttp://hdl.handle.net/10198/35722
dc.language.isoeng
dc.peerreviewedyes
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectApis mellifera
dc.titleGenetic variation of detoxification genes: from genes to proteinseng
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferenceDate2025
oaire.citation.conferencePlaceCopenhagen, Denmark
oaire.citation.startPage64
oaire.citation.titleApimondia 2025
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameHenriques
person.familyNameLi
person.familyNameQuaresma
person.familyNameLopes
person.familyNameShiraishi
person.familyNameYadró Garcia
person.familyNameAbreu
person.familyNameRufino
person.familyNamePinto
person.givenNameDora
person.givenNameFernanda
person.givenNameAndreia
person.givenNameAna Rita
person.givenNameCarlos S.H.
person.givenNameCarlos A.
person.givenNameRui M.V.
person.givenNameJosé
person.givenNameM. Alice
person.identifier.ciencia-id291F-986F-07DA
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person.identifier.ciencia-id6C11-86D5-73AE
person.identifier.ciencia-idF71F-B08E-EC39
person.identifier.ciencia-id0F19-0DE2-12A2
person.identifier.ciencia-idC414-F47F-6323
person.identifier.ciencia-idF814-A1D0-8318
person.identifier.orcid0000-0001-7530-682X
person.identifier.orcid0000-0003-0466-355X
person.identifier.orcid0000-0002-8678-5800
person.identifier.orcid0000-0002-1888-2262
person.identifier.orcid0000-0002-4174-8985
person.identifier.orcid0000-0002-6916-3647
person.identifier.orcid0000-0002-7745-8015
person.identifier.orcid0000-0002-1344-8264
person.identifier.orcid0000-0001-9663-8399
person.identifier.scopus-author-id55761737300
person.identifier.scopus-author-id57119742600
person.identifier.scopus-author-id7003290613
person.identifier.scopus-author-id55947199100
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