Orientador(es)
Resumo(s)
Laimaphelenchus suberensis sp. nov. obtained
from declining Quercus suber trees of Herdade da
Gouveia de Baixo, Alentejo, Portugal, is described and
illustrated based on morphological, biometrical and molecular
characters. The diagnosis of Laimaphelenchus
species has been commonly based on the presence or
absence of a vulval flap and on the shape structure of the
tail tip. The species described here has been included in
the Laimaphelenchus group without vulval flap, and can
be distinguished from morphologically similar species
by its tail tip shape structure that has a stalk-like terminus
and three diffuse tubercles with 4–6 finger-like
protrusions. For the molecular analyses, the mitochondrial
DNA region from the cytochrome oxidase subunit
I (mtCOI), the D2-D3 expansion segments of the large
subunit (LSU) and small subunit (SSU) of rRNA gene
were amplified and sequenced. Sequences of
L. suberensis sp. nov. clustered separately from all
Laimaphelenchus spp. with available sequences in
Genbank, confirming its identification as a new species.
This is the second report of the genus Laimaphelenchus
in Portugal, associated with Q. suber: L. heidelbergi and
L. suberensis sp. nov.
Descrição
Palavras-chave
Cork oak Cytochrome oxidase subunit I Large subunit ribosomal RNA Molecular analysis Morphology Small subunit ribosomal RNA
Contexto Educativo
Citação
Maleita, Carla Maria Nobre; Costa, Sofia R.; Abrantes, Isabel (2018). Laimaphelenchus suberensis sp. nov. associated with Quercus suber in Portugal. European Journal of Plant Pathology. ISSN 0929-1873. 150, p. 747-758