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Characterization of chestnut bark fungal communities in healthy trees and blight recovered through natural or introduced hypovirulence

2023Journal article Open access
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dc.contributor.authorCoelho, Valentim
dc.contributor.authorNunes, Luís
dc.contributor.authorMoura, Luísa
dc.contributor.authorGouveia, Maria Eugénia
dc.date.accessioned2023-10-20T10:40:32Z
dc.date.available2023-10-20T10:40:32Z
dc.date.issued2023
dc.description.abstractVarious fungal species together with Cryphonectria parasitica (Murr.) Barr. have been isolated from chestnut tissues with blight symptoms. Microfungi remain in cankers during tissue healing, which occurs by transmitting hypovirulence by Cryphonectria hypovirus 1 (CHV1) compatible strains. However, studies focused on the diversity and ecology of the non-C. parasitica taxa on chestnut bark are lacking. This work evaluated the composition and richness of microfungi species associated with healthy chestnut trees, those with cankers healed by natural hypovirulence and those treated with hypovirulent strains (artificially introduced hypovirulence). Microfungi from diseased trees were isolated from six randomly selected points in the inner and external areas of the healed canker. In healthy trees, tissue samples were collected from 12 random locations on each tree’s trunk. Fungal species were identified based on morphological characteristics and ITS region sequencing using the universal primers ITS1 and ITS4. Four hundred thirty-one fungal isolates were obtained from which 38 operational taxonomic units (OTUs) were identified. The fungal communities varied from tree to tree and did not display similar patterns. The endophyte Biscogniauxia mediterranea and epiphyte Cytospora eucalypticola fungi were detected in all study locations and tree health conditions. Notably, C. parasitica (virulent and hypovirulent strains) was dominant in the inner area of healed cankers, accounting for 64.3% of the isolates, and the saprobe fungi Penicillium glabrum was dominant among non-C. parasitica microfungi species. Dissimilarity analyses showed low similarity between the microfungi communities found in the inner and external areas of the healed cankers. The study reveals the long-life span of C. parasitica in healed cankers and the therapeutic effect of natural and introduced hypovirulence.pt_PT
dc.description.sponsorshipThis study was funded by the Project BioChestnut PDR2020-101-030959pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCoelho, Valentim; Nunes, Luís; Moura, Luísa; Gouveia, Eugénia (2023). Characterization of chestnut bark fungal communities in healthy trees and blight recovered through natural or introduced hypovirulence. Journal of Plant Pathology. ISSN 1125-4653. 105:3, p. 1031-1043pt_PT
dc.identifier.doi10.1007/s42161-023-01424-ypt_PT
dc.identifier.issn1125-4653
dc.identifier.urihttp://hdl.handle.net/10198/28802
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Naturept_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectHealed cankerspt_PT
dc.subjectMicrofungal communitiespt_PT
dc.subjectCryphonectria parasiticapt_PT
dc.subjectBiscogniauxia mediterraneapt_PT
dc.subjectPezicula ericaept_PT
dc.titleCharacterization of chestnut bark fungal communities in healthy trees and blight recovered through natural or introduced hypovirulencept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1043pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage1031pt_PT
oaire.citation.titleJournal of Plant Pathologypt_PT
oaire.citation.volume105pt_PT
person.familyNameCoelho
person.familyNameNunes
person.familyNameGouveia
person.givenNameValentim
person.givenNameLuís
person.givenNameMaria Eugénia
person.identifier.ciencia-id0618-49BC-EACE
person.identifier.orcid0000-0001-5829-7669
person.identifier.orcid0000-0002-8913-785X
person.identifier.orcid0000-0002-2550-9108
person.identifier.scopus-author-id26533798200
person.identifier.scopus-author-id35222136600
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication99a97d58-8b4c-4a49-9855-d334d568b9f3
relation.isAuthorOfPublicatione5a7d07d-f55c-4068-a4ec-258574539652
relation.isAuthorOfPublication99e4701b-e3c2-4594-a37f-42bd49821d6e
relation.isAuthorOfPublication.latestForDiscovery99e4701b-e3c2-4594-a37f-42bd49821d6e

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