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Cloning and expression analysis of glucanase genes from Phytophthora cinnamomi

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dc.contributor.authorMartins, Ivone
dc.contributor.authorMeirinho, Sofia G.
dc.contributor.authorDias, Teresa
dc.contributor.authorJorge, Lurdes
dc.contributor.authorMartins, Fátima
dc.contributor.authorChoupina, Altino
dc.date.accessioned2011-02-14T09:55:10Z
dc.date.available2011-02-14T09:55:10Z
dc.date.issued2010
dc.description.abstractPhytophthora cinnamomi is one among the most destructive species of Phytophthora associated to the decline of forestry, ornamental and fruit species. Associated with this oomycete is the ink disease of Castanea sativa Mill. Glucan endo-1,3-β-D-glucosidase catalyzes the hydrolysis of 1,3-β-D-glucoside linkages in callose, laminarin and several carbohydrates found in the cell wall of plants and fungi. It is generally thought that glucanases play a role in plant defence by digesting wall components of the fungal pathogen. In oomycetes, glucanases have been studied at biochemical level for their possible role in hyphal tip growth and branching, where there is thought to be a delicate balance between the cell wall synthesis and hydrolysis. Fungal cell wall degrading enzyme production is influenced by a number of factors including the type of strain, the culture conditions and substrate type. The aim of this work was the analysis of homologous expression, in P. cinnamomi, and heterologous expression, in Pichia pastoris, of the endo-1,3-β-D-glucosidase encoding gene ENDO1 produced by P. cinnamomi. The expression was studied during growth in different carbon sources and was also performed a time course of endo-1,3-β-D-glucosidase production. Different plasmids were used to clone the gene on each organism and we used RT-PCR analysis to examine its expression. The major expression levels occurred at the medium with glucose as carbon source. These and other results will be presented.por
dc.identifier.citationMartins, Ivone; Meirinho, Sofia; Dias, Teresa; Jorge, Lurdes; Martins, Fátima; Choupina, Altino (2010). Cloning and expression analysis of glucanase genes from Phytophthora cinnamomi. In 9th Conference of the European Foundation for Plant Pathology. 6th Congress of the Sociedade Portuguesa de Fitopatologia. Évorapor
dc.identifier.urihttp://hdl.handle.net/10198/3369
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSociedade Portuguesa de Fitopatologiapor
dc.relationIdentificação, caracterização e papel de factores moleculares associados ao mecanismo de infecção de espécies de Fagaceae por Phytophthora cinnamomi
dc.subjectCastanea sativa Mill.por
dc.subjectENDO1por
dc.subjectHomologous expressionpor
dc.subjectHeterologous expressionpor
dc.titleCloning and expression analysis of glucanase genes from Phytophthora cinnamomipor
dc.typeconference object
dspace.entity.typePublication
oaire.awardNumberPTDC/AGR-AAM/67628/2006
oaire.awardTitleIdentificação, caracterização e papel de factores moleculares associados ao mecanismo de infecção de espécies de Fagaceae por Phytophthora cinnamomi
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-AAM%2F67628%2F2006/PT
oaire.citation.conferencePlaceÉvorapor
oaire.citation.endPage181por
oaire.citation.startPage181por
oaire.citation.title9th Conference of the European Foundation for Plant Pathology. 6th Congress of the Sociedade Portuguesa de Fitopatologiapor
oaire.fundingStream3599-PPCDT
person.familyNameDias
person.familyNameJorge
person.familyNameChoupina
person.givenNameTeresa
person.givenNameLurdes
person.givenNameAltino
person.identifier142703
person.identifier587972
person.identifier.ciencia-idFF1F-B010-1399
person.identifier.ciencia-id1A14-77FC-9656
person.identifier.orcid0000-0002-9419-9561
person.identifier.orcid0000-0003-0141-0995
person.identifier.orcid0000-0002-3956-9398
person.identifier.ridL-5382-2014
person.identifier.scopus-author-id56830642600
person.identifier.scopus-author-id14051602500
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typeconferenceObjectpor
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relation.isAuthorOfPublicationfe4754f2-bf17-4663-9707-07ba3260cf74
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