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Advances in isoflavone profile characterisation using matrix solid-phase dispersion coupled to HPLC/DAD in medicago species

2015Journal article Open access
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dc.contributor.authorVisnevschi-Necrasov, Tatiana
dc.contributor.authorBarreira, João C.M.
dc.contributor.authorCunha, Sara C.
dc.contributor.authorPereira, Graça
dc.contributor.authorNunes, Eugénia
dc.contributor.authorOliveira, Beatriz
dc.date.accessioned2018-02-19T10:00:00Z
dc.date.accessioned2018-03-01T16:05:02Z
dc.date.accessioned2018-03-05T09:18:52Z
dc.date.available2018-01-19T10:00:00Z
dc.date.available2018-03-01T16:05:02Z
dc.date.available2018-03-05T09:18:52Z
dc.date.issued2015
dc.description.abstractAnalytical methods used in phytochemistry analysis are limited by the sample preparation step, which should ideally be fast, accurate, ecofriendly and achievable using low quantities of the sample. Matrix solid-phase dispersion (MSPD) may be a good alternative for combining extraction and purification procedures, thereby reducing the indicated limitations. Objective-Applying an MSPD extraction procedure coupled to high-performance liquid chromatography diode-array detection (HPLC/DAD) as an alternative methodology to evaluate isoflavone profiles. Methods-Isoflavone profiles were determined for the leaves of nine species of Medicago in the late flower phenological stage (one or more nodes with 50% open flowers, no seed pods). Extraction was performed following MSPD, and isoflavone profiles were characterised using HPLC/DAD. The quantified amounts were compared with previous results in different species commonly recognised as good sources of isoflavones. Results-Formononetin was the major isoflavone in most species, except M. polymorpha and M. truncatula. The isoflavone amounts were significantly different among the assayed species, with M. orbicularis and M. arabica as the major isoflavone sources, while M. rigidula presented the lowest contents. Furthermore, the detected differences allow electing the best species as a primary source of a specific isoflavone. Conclusion-The MSPD allowed good extraction efficiency, reproducibility and recovery. Some of the species showed relevant isoflavone contents, even when compared with acknowledged plant sources such as soy or red clover. To the best of our knowledge the results presented are reported for the first time in these species.en_EN
dc.description.sponsorshipT. Visnevschi-Necrasov and João C.M. Barreira are grateful to ‘FCT - Fundação para a Ciência e a Tecnologia’ for their grants (BD/ 31734/2006 and SFRH/BPD/72802/2010, respectively). S.C. Cunha is grateful to ‘POPH-QREN - Tipologia 4.2, Fundo Social Europeu e Fundo Nacional MCTES’.
dc.description.versioninfo:eu-repo/semantics/publishedVersionen_EN
dc.identifier.citationVisnevschi-Necrasov, Tatiana; Barreira, Jõo C.M.; Cunha, Sara C.; Pereira, Graça; Nunes, Eugénia; Oliveira, M. Beatriz P.P. (2015). Advances in isoflavone profile characterisation using matrix solid-phase dispersion coupled to HPLC/DAD in medicago species. Phytochemical Analysis. ISSN 0958-0344. 26, p. 40-46en_EN
dc.identifier.doi10.1002/pca.2534en_EN
dc.identifier.issn0958-0344
dc.identifier.urihttp://hdl.handle.net/10198/16130
dc.language.isoeng
dc.peerreviewedyesen_EN
dc.relationBIOACTIVE PROPERTIES & CYTOPROTECTIVE POTENTIAL OF NATURAL EXTRACTS/INDIVIDUAL COMPOUNDS: APPLICATION OF SINGLE CELL GEL ELECTROPHORESIS AND OTHER BIOCHEMICAL, CHEMICAL AND ELECTROCHEMICAL ASSAYS
dc.subjectHPLC/DADen_EN
dc.subjectInterspecific variationen_EN
dc.subjectIsoflavonesen_EN
dc.subjectMedicagoen_EN
dc.subjectMSPDen_EN
dc.titleAdvances in isoflavone profile characterisation using matrix solid-phase dispersion coupled to HPLC/DAD in medicago speciesen_EN
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBIOACTIVE PROPERTIES & CYTOPROTECTIVE POTENTIAL OF NATURAL EXTRACTS/INDIVIDUAL COMPOUNDS: APPLICATION OF SINGLE CELL GEL ELECTROPHORESIS AND OTHER BIOCHEMICAL, CHEMICAL AND ELECTROCHEMICAL ASSAYS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F31734%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F72802%2F2010/PT
oaire.fundingStreamSFRH
person.familyNameBarreira
person.givenNameJoão C.M.
person.identifier.orcid0000-0003-1233-0990
person.identifier.ridD-8269-2013
person.identifier.scopus-author-id54895546900
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccessen_EN
rcaap.typearticleen_EN
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relation.isAuthorOfPublication.latestForDiscovery4629b12c-39b0-4da8-8b8d-6efba5cf2d81
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