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Optimization of ultrasound-assisted extraction to obtain mycosterols from Agaricus bisporus L. by response surface methodology and comparison with conventional Soxhlet extraction

2016Journal article Open access
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dc.contributor.authorHeleno, Sandrina A.
dc.contributor.authorDiz, Patrícia do Carmo Claro
dc.contributor.authorPrieto Lage, Miguel A.
dc.contributor.authorBarros, Lillian
dc.contributor.authorRodrigues, Alírio
dc.contributor.authorBarreiro, M.F.
dc.contributor.authorFerreira, Isabel C.F.R.
dc.date.accessioned2016-09-29T09:19:35Z
dc.date.available2016-09-29T09:19:35Z
dc.date.issued2016
dc.description.abstractErgosterol, a molecule with high commercial value, is the most abundant mycosterol in Agaricus bisporus L. To replace common conventional extraction techniques (e.g. Soxhlet), the present study reports the optimal ultrasound-assisted extraction conditions for ergosterol. After preliminary tests, the results showed that solvents, time and ultrasound power altered the extraction efficiency. Using response surface methodology, models were developed to investigate the favourable experimental conditions that maximize the extraction efficiency. All statistical criteria demonstrated the validity of the proposed models. Overall, ultrasound-assisted extraction with ethanol at 375 W during 15 min proved to be as efficient as the Soxhlet extraction, yielding 671.5 ± 0.5mg ergosterol/100 g dw. However, with n-hexane extracts with higher purity (mg ergosterol/g extract) were obtained. Finally, it was proposed for the removal of the saponification step, which simplifies the extraction process and makes it more feasible for its industrial transference.pt_PT
dc.description.sponsorshipThe authors acknowledge FCT (Portugal) for financial support to CIMO (PEst-OE/AGUI0690/2014), Sandrina A. Heleno (SFRH/BPD/101413/2014) and L. Barros research contract, to FCT/MEC and FEDER under Programme PT2020 for financial support to LSRE (Project UID/EQU/50020/2013), and to QREN, ON2 and FEDER (Projects NORTE-07-0124-FEDER-000014 and NORTE-07-0162-FEDER-000050). Xunta de Galicia for financial support for the post-doctoral researcher of M.A. Prieto.pt_PT
dc.identifier.citationHeleno, Sandrina A.; Diz, Patrícia; Prieto, M.A.; Barros, Lillian; Rodrigues, A.E.; Barreiro, M.F.; Ferreira, Isabel C.F.R. (2016). Optimization of ultrasound-assisted extraction to obtain mycosterols from Agaricus bisporus L. by response surface methodology and comparison with conventional Soxhlet extraction. Food Chemistry. ISSN 0308-8146. 197, p. 1054-1063pt_PT
dc.identifier.doi10.1016/j.foodchem.2015.11.108pt_PT
dc.identifier.issn0308-8146
dc.identifier.urihttp://hdl.handle.net/10198/13332
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationObtaining of mycosterols to develop new functionalized foods: extraction, refinement, stabilization and incorporation into dairy products
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAgaricuspt_PT
dc.subjectChemical fractionationpt_PT
dc.subjectHexanespt_PT
dc.subjectSterolspt_PT
dc.subjectUltrasonicspt_PT
dc.titleOptimization of ultrasound-assisted extraction to obtain mycosterols from Agaricus bisporus L. by response surface methodology and comparison with conventional Soxhlet extractionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleObtaining of mycosterols to develop new functionalized foods: extraction, refinement, stabilization and incorporation into dairy products
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/PEst-OE%2FAGR%2FUI0690%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F101413%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FEQU%2F50020%2F2013/PT
oaire.citation.endPage1063pt_PT
oaire.citation.startPage1054pt_PT
oaire.citation.titleFood Chemistrypt_PT
oaire.citation.volume197pt_PT
oaire.fundingStream5876
oaire.fundingStream5876
person.familyNameHeleno
person.familyNamePrieto Lage
person.familyNameBarros
person.familyNameBarreiro
person.familyNameFerreira
person.givenNameSandrina A.
person.givenNameMiguel A.
person.givenNameLillian
person.givenNameFilomena
person.givenNameIsabel C.F.R.
person.identifier107688
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person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0001-7224-1098
person.identifier.orcid0000-0002-3513-0054
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0002-6844-333X
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridL-5951-2014
person.identifier.ridG-4516-2011
person.identifier.ridJ-3600-2013
person.identifier.ridL-9802-2014
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id30067731800
person.identifier.scopus-author-id35937495700
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id57188719717
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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