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Analytical validation of an ultraviolet–visible procedure for determining vitamin D3 in vitamin D3-loaded microparticles and toxigenetic studies for incorporation into food

2021Journal article Open access
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dc.contributor.authorSilva, Tamires Barlati Vieira
dc.contributor.authorOliveira, Anielle
dc.contributor.authorMoreira, Thaysa Fernandes Moya
dc.contributor.authorSilva, Kelly Cristina
dc.contributor.authorZanin, Rodolfo Campos
dc.contributor.authorBona, Evandro
dc.contributor.authorGonçalves, Odinei Hess
dc.contributor.authorShirai, Marianne Ayumi
dc.contributor.authorPeron, Ana Paula
dc.contributor.authorLeimann, Fernanda Vitória
dc.date.accessioned2018-02-19T10:00:00Z
dc.date.accessioned2021-11-22T14:46:25Z
dc.date.available2018-01-19T10:00:00Z
dc.date.available2021-11-22T14:46:25Z
dc.date.issued2021
dc.description.abstractVitamin D is a water-insoluble compound presented in two main forms (D2 and D3), susceptible to environmental conditions. Microencapsulation is an alternative to supplements and preserve vitamin D properties in foods. Entrapment efficiency (EE) is the main property to evaluate the encapsulation effectiveness and therefore it is of interest the study of analytical methods for the identification and quantification of this compound within the particle. This paper describes a low cost UV–Vis methodology validation to the identification and quantification of vitamin D3 in microparticles produced by hot homogenization. The method was validated following the International Conference on Harmonization (ICH) guidelines. To guarantee safe application in foodstuff, microparticles toxigenicity was evaluated with Allium cepa L. in vivo model, showing no cytotoxic nor genotoxic potential. High entrapment efficiency was obtained, the results also demonstrated that the concentration of vitamin D3 in microparticles can be safely accessed by the validated method.en_EN
dc.description.sponsorshipThis study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code 001. The authors thank the Multiuser Laboratory of Federal University of Technology – Paraná – Campus Londrina, and the “Central Analítica Multiusuário da UTFPR Campo Mourão” (CAMulti-CM) for the analyses. Also, to CNPq for the financial support (Project n◦ 420055/2018-5) and scholarship.
dc.description.versioninfo:eu-repo/semantics/publishedVersionen_EN
dc.identifier.citationSilva, Tamires Barlati Vieira da; Oliveira, Anielle de; Moreira, Thaysa Fernandes Moya; Silva, Kelly Cristina de; Zanin, Rodolfo Campos; Bona, Evandro; Gonçalves, Odinei Hess; Shirai, Marianne Ayumi; Peron, Ana Paula; Leimann, Fernanda Vitória (2021). Analytical validation of an ultraviolet–visible procedure for determining vitamin D3 in vitamin D3-loaded microparticles and toxigenetic studies for incorporation into food. Food Chemistry. ISSN 0308-8146. p. 1-7en_EN
dc.identifier.doi10.1016/j.foodchem.2021.129979en_EN
dc.identifier.issn0308-8146
dc.identifier.urihttp://hdl.handle.net/10198/24259
dc.language.isoeng
dc.peerreviewedyesen_EN
dc.subjectCarnauba waxen_EN
dc.subjectCytotoxicityen_EN
dc.subjectEncapsulationen_EN
dc.subjectGenotoxicityen_EN
dc.subjectHot homogenizationen_EN
dc.subjectSpectroscopyen_EN
dc.subjectVitaminen_EN
dc.titleAnalytical validation of an ultraviolet–visible procedure for determining vitamin D3 in vitamin D3-loaded microparticles and toxigenetic studies for incorporation into fooden_EN
dc.typejournal article
dspace.entity.typePublication
person.familyNameGonçalves
person.familyNameLeimann
person.givenNameOdinei Hess
person.givenNameFernanda Vitória
person.identifier.ciencia-id8F10-A04D-FC0D
person.identifier.orcid0000-0002-9528-8187
person.identifier.orcid0000-0001-6230-9597
person.identifier.scopus-author-id9335958400
rcaap.rightsopenAccessen_EN
rcaap.typearticleen_EN
relation.isAuthorOfPublication84c8eea0-77bb-4c18-b9d8-46f865980706
relation.isAuthorOfPublicationb1102a0b-38dc-4caa-baed-ad52ccd9b230
relation.isAuthorOfPublication.latestForDiscovery84c8eea0-77bb-4c18-b9d8-46f865980706

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