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Advisor(s)
Abstract(s)
To respond to the increasing global demand for fish, nowadays, almost 50% of the
global fish market comes from aquaculture production [1]. Thus, there is the need to
assure a correct information, not only about the species, but also about the
production method (farmed vs. wild) and the catch origin of fish. Salmon, a hightrophic-
level carnivorous species with high economic value due to its popularity, is
among the fish species that is frequently produced in aquaculture. Although the feed
given to farm-raised salmon is designed to meet its nutritional requirements, it can
present differences compared to the diet of wild salmon that can be reflected on the
muscle composition of farmed versus wild salmons. Therefore, this work aims at
comparing the fatty acid composition of salmon from aquaculture and caught in the
wild.
Salmon specimens caught in the wild (n = 25) and farm-raised (n = 25) were
obtained from West of Vancouver Island and Campbell River (Canada), respectively.
Two lipid extraction methods (Soxhlet extraction with n-hexane and an adaptation of
the Bligh and Dyer extraction method) and two derivatization procedures (alkaline
transmethylation using KOH and acid-catalyzed transmethylation using BF3/MEOH
solution) were tested. Fatty acid methyl esters (FAME) were analyzed in a Shimadzu
GC-2010 Plus gas chromatograph equipped with a Shimadzu AOC-20i auto-injector,
a flame ionization detector and a CP-Sil 88 silica capillary column (50 x 0.25 mm i.d.,
0.20 μm). The injector and detector temperatures were 250 and 270 °C, respectively.
The compounds were identified by comparison with standards (FAME 37, Supelco).
Based on the obtained results, the modified Bligh and Dyer method was chosen for
lipid extraction since it allowed obtaining higher amounts of long chain unsaturated
fatty acids, particularly of docosahexaenoic acid (DHA). Similar results were obtained
for both tested derivatization methodologies. In general, the two groups of salmon
samples showed different profiles, with wild samples presenting significantly higher
contents of omega-3 fatty acids, in particular docosahexaenoic and eicosapentaenoic
acids, while farmed salmon had higher amounts of oleic and linoleic acids.
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Citation
Grazina, Liliana; Nunes, Maria; Mafra, Isabel; Oliveira, M.B.P.P.; Amaral, J.S. (2018). Comparative analysis of fatty acid composition of wild vs. farmed salmon. In 9th Meeting of Division of Analytical Chemistry (ANALÍTICA 2018). Porto