Orientador(es)
Resumo(s)
Since annually a high amount of wastes is produced in vine pruning, the aimof this studywas to evaluate the potential
of vine shoots fromtwo Portuguese grape varieties (Touriga Nacional - TNand Tinta Roriz - TR) to be used
as a natural source of phenolic compounds. To reach this goal, three techniques were explored, namely
microwave-assisted extraction (MAE), subcritical water extraction (SWE) and conventional extraction (CE).
The phenolic composition of the extracts, antioxidant and biological activities were evaluated by spectrophotometry
and chromatography. MAE and SWE produced the highest concentrated extracts. TR vine shoot variety had
the highest antioxidant activity and total phenolic (32.1±0.9mggallic acid equivalents/g dry sample), as well as
flavonoid content (18.7±1.2mg epicatechin equivalents/g dry sample). For the first time, the biological activity
of the vine shoot extracts was tested. Results demonstrated that all of them had antimicrobial potential against
different bacteria and yeasts, and the ability of inhibiting α-amylase and acetylcholinesterase enzymes, with
MAE TR extracts being the most efficient. HPLC analysis enabled the identification of different phenolic compounds, with gallic acid, catechin, myricetin and kaempferol-3-O-rutinoside being the main contributors to
the phenolic composition. Portuguese vine shootwastes could serve as easily accessible source of natural antioxidants
for the food or pharmaceutical industries.
Descrição
Palavras-chave
Antimicrobial activity Antioxidant activity Bioactive compounds HPLC-PDA analysis Polyphenols Vine shoots
Contexto Educativo
Citação
Moreira, Manuela M.; Barroso, M. Fátima; Porto, João Vasconcellos; Ramalhosa, M. J.; Švarc-Gajić, Jaroslava; Estevinho, Leticia; Morais, Simone; Delerue-Matos, Cristina (2018). Potential of Portuguese vine shoot wastes as natural resources of bioactive compounds. Science of the Total Environment. ISSN 0048-9697. 634, p. 831-842