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Assessment of Trioza erytreae microbiome and mitochondrial genome variability by integrated high-throughput sequencing approach

2025Journal article Open access
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datacite.subject.fosCiências Naturais::Ciências Biológicas
datacite.subject.fosCiências Naturais::Outras Ciências Naturais
datacite.subject.fosEngenharia e Tecnologia::Biotecnologia Ambiental
datacite.subject.fosEngenharia e Tecnologia::Outras Engenharias e Tecnologias
datacite.subject.sdg02:Erradicar a Fome
datacite.subject.sdg15:Proteger a Vida Terrestre
dc.contributor.authorChiumenti, Michela
dc.contributor.authorNicoloso, Vittorio
dc.contributor.authorFereres, Alberto
dc.contributor.authorPereira, José Alberto
dc.contributor.authorMaree, Hans J.
dc.contributor.authorBester, Rachelle
dc.contributor.authorReynaud, Bernard
dc.contributor.authorDelatte, Hélène
dc.contributor.authorPeña, Leandro
dc.contributor.authorPallás, Vicente
dc.contributor.authorSerra, Pedro
dc.contributor.authorNavarro, Beatriz
dc.contributor.authorSerio, Francesco Di
dc.date.accessioned2025-12-05T11:16:19Z
dc.date.available2025-12-05T11:16:19Z
dc.date.issued2025
dc.description.abstractHuanglongbing, a devastating citrus disease, is associated with 'Candidatus Liberibacter asiaticus', 'Ca. L. africanus' or 'Ca L. americanus', bacteria transmitted by the psylloids Diaphorina citri and Trioza erytreae. Using a DNA-Seq and metabarcode sequencing integrated approach, the first catalogue of endosymbionts associated with T. erytreae from the Iberian Peninsula, South Africa and African Islands, was generated. The almost complete genome of two new bacteria, one facultative and one obligate, tentatively named Asaia-like endosymbiont of T. erytreae and Sodalis-like endosymbiont of T. erytreae, respectively, was assembled and annotated. The complete mitochondrial genomes of T. erytreae from the geographical areas studied were also assembled and phylogenetic analyses were performed, suggesting that T. erytreae populations currently present in the Iberian Peninsula and specimens analyzed from South Africa may have originated from a common ancestor. Similar results were obtained when the genetic distances between Sodalis-like endosymbiont of T. erytreae were taken into consideration, thus supporting the symbiont-host codivergence which suggests that this bacterium is approaching to an obligate status. Finally, a new genetic marker of T. erytreae, an insertion in the mitochondrial tRNA-Ser gene, was identified only in some European samples, showing for the first time the existence of two mixed subpopulations of T. erytreae. The integrated DNA-Seq and metabarcode sequencing approach used in this study, besides generating a catalogue of T. erytreae endosymbionts, provided novel data on the sequence variability of bacterial and insect mitochondrial genomes from different geographic areas, highlighting the possible original sources of currently spreading T. erytreae populations may be more complex than previously reported.eng
dc.description.sponsorshipOpen access funding provided by Consiglio Nazionale Delle Ricerche (CNR) within the CRUI-CARE Agreement. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 817526 (PRE-HLB: Preventing HLB epidemics for ensuring citrus survival in Europe. H2020-SFS-2018–2 Topic SFS-05–2018–2019–2020—new and emerging risks to plant health). This article reflects only the authors’ views, and the Agency is not responsible for any use that may be made of the information it contains.
dc.identifier.citationChiumenti, Michaela; Nicoloso, Vittorio; Fereres, Alberto; Pereira, Jose A.; Maree, Hans J.; Bester, Rachelle; Reynaud, Bernard; Delatte, Helene; Pena, Leandro; Pallas, Vicente; Serra, Pedro; Navarro, Beatriz; Di Serio, Francesco. (2025). Assessment of Trioza erytreae microbiome and mitochondrial genome variability by integrated high-throughput sequencing approach. Journal of Pest Science. ISSN 1612-4766. 98:4, p. 2383-2402.
dc.identifier.doi10.1007/s10340-025-01945-8
dc.identifier.issn1612-4758
dc.identifier.issn1612-4766
dc.identifier.urihttp://hdl.handle.net/10198/35183
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer Science and Business Media LLC
dc.relation.ispartofJournal of Pest Science
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectAfrican citrus triozid
dc.subjectEndosymbiont
dc.subjectPhylogeography
dc.subjectDNA-Seq
dc.subject16S rRNAs
dc.subjectHaplotype
dc.titleAssessment of Trioza erytreae microbiome and mitochondrial genome variability by integrated high-throughput sequencing approacheng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage2402
oaire.citation.issue4
oaire.citation.startPage2383
oaire.citation.titleJournal of Pest Science
oaire.citation.volume98
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNamePereira
person.givenNameJosé Alberto
person.identifier.ciencia-id611F-80B2-A7C1
person.identifier.orcid0000-0002-2260-0600
person.identifier.ridL-6798-2014
person.identifier.scopus-author-id57204366348
relation.isAuthorOfPublication7932162e-a2da-4913-b00d-17babbe51857
relation.isAuthorOfPublication.latestForDiscovery7932162e-a2da-4913-b00d-17babbe51857

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