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Protective effect of electron beam irradiation in tocopherols integrity: evaluation by HPLC-fluorescence detection

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dc.contributor.authorFernandes, Ângela
dc.contributor.authorBarreira, João C.M.
dc.contributor.authorAntonio, Amilcar L.
dc.contributor.authorMartins, Anabela
dc.contributor.authorOliveira, Beatriz
dc.contributor.authorFerreira, Isabel C.F.R.
dc.date.accessioned2014-03-27T12:23:39Z
dc.date.available2014-03-27T12:23:39Z
dc.date.issued2013
dc.description.abstractThe high perishability is a common characteristic in mushrooms. This limitation demands for continuous investigation to achieve an effective conservation technology, allowing their preservation while simultaneously protecting the main bioactive compounds and the chemical composition in general. However, these two objectives might be more accurately achieved by combining two technologies, instead of using a single one. Therefore, electron-beam irradiation (up to 10 kGy) was applied to dried samples of Boletus edulis and Russula delica, increasing previous knowledge using gamma- and electron-beam irradiation at lower doses (up to 6 kGy) and different wild mushroom species. In this study, an HPLC system coupled to a fluorescence detector (FP-2020; Jasco), programmed for excitation at 290 nm and emission at 330 nm, was used to evaluate changes in tocopherols profile among irradiated and non-irradiated mushrooms. The chromatographic separation was achieved with a Polyamide 11 normal phase column (250 x 4.6 mm; YMC Waters) operating at 30 °C. The mobile phase used was a mixture of n-hexane and ethyl acetate (70:30, v/v) at a flow rate of 1 ml/min. The compounds were identified by chromatographic comparisons with authentic standards. Quantification was based on the fluorescence signal response, using the internal standard method. The vitamer quantified in highest amount was δ-tocopherol in both mushrooms, but B. edulis presented higher total tocopherols quantities. The effects on tocopherols profiles were significant for all quantified isoforms (except δ-tocopherol in B. edulis). Irradiated samples tended to present higher amounts, as it was particularly observed for 2 kGy dose in B. edulis (129 μg /100 g dw) and the 6 kGy in R. delica (87 μg /100 g dw), showing consistency with a previous study [1]. This result might be explained by differences in free oxygen availability inside the polyethylene bag. Overall, the applied doses might guarantee not only desinfested and decontaminated samples, but also provide the aditional advantage of protecting one of the important bioactive lipophilic compounds.por
dc.identifier.citationFernandes, Ângela; Barreira, João C.M.; Antonio, Amilcar L.; Martins, Anabela; Oliveira, M. Beatriz P.P.; Ferreira, Isabel C.F.R. (2013). Protective effect of electron beam irradiation in tocopherols integrity: evaluation by HPLC-fluorescence detection. In 8° Encontro Nacional de Cromatografia. Covilhã: Universidade da Beira Interior. ISBN 978-989-98541-1-6por
dc.identifier.isbn978-989-98541-1-6
dc.identifier.urihttp://hdl.handle.net/10198/9390
dc.language.isoengpor
dc.peerreviewedyespor
dc.relationBIOACTIVE PROPERTIES & CYTOPROTECTIVE POTENTIAL OF NATURAL EXTRACTS/INDIVIDUAL COMPOUNDS: APPLICATION OF SINGLE CELL GEL ELECTROPHORESIS AND OTHER BIOCHEMICAL, CHEMICAL AND ELECTROCHEMICAL ASSAYS
dc.relationStrategic Project - UI 690 - 2011-2012
dc.relationStrategic Project - LA 6 - 2011-2012
dc.titleProtective effect of electron beam irradiation in tocopherols integrity: evaluation by HPLC-fluorescence detectionpor
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleBIOACTIVE PROPERTIES & CYTOPROTECTIVE POTENTIAL OF NATURAL EXTRACTS/INDIVIDUAL COMPOUNDS: APPLICATION OF SINGLE CELL GEL ELECTROPHORESIS AND OTHER BIOCHEMICAL, CHEMICAL AND ELECTROCHEMICAL ASSAYS
oaire.awardTitleStrategic Project - UI 690 - 2011-2012
oaire.awardTitleStrategic Project - LA 6 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F76019%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F72802%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FAGR%2FUI0690%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FEQB%2FLA0006%2F2011/PT
oaire.citation.conferencePlaceCovilhã, Portugalpor
oaire.citation.title8º Encontro Nacional de Cromatografiapor
oaire.fundingStreamSFRH
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6820 - DCRRNI ID
person.familyNameFernandes
person.familyNameBarreira
person.familyNameAntonio
person.familyNameMartins
person.familyNameFerreira
person.givenNameÂngela
person.givenNameJoão C.M.
person.givenNameAmilcar L.
person.givenNameAnabela
person.givenNameIsabel C.F.R.
person.identifier144781
person.identifier.ciencia-id2015-0434-AE7C
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person.identifier.orcid0000-0002-0157-9873
person.identifier.orcid0000-0003-1233-0990
person.identifier.orcid0000-0001-8271-9964
person.identifier.orcid0000-0001-6218-4413
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridD-8269-2013
person.identifier.ridJ-1960-2014
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person.identifier.ridE-8500-2013
person.identifier.scopus-author-id54895546900
person.identifier.scopus-author-id44560921900
person.identifier.scopus-author-id7203013518
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
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