Repository logo
 
Publication

Clitocybe alexandri extract induces apoptosis in a lung cancer cell line: identification of phenolic acids with cytotoxic potential

2011Conference object Open access
Simple item page
dc.contributor.authorVaz, Josiana A.
dc.contributor.authorAlmeida, Gabriela M.
dc.contributor.authorFerreira, Diana G.
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorMartins, Anabela
dc.contributor.authorVasconcelos, M. Helena
dc.date.accessioned2011-08-23T14:22:22Z
dc.date.available2011-08-23T14:22:22Z
dc.date.issued2011
dc.description.abstractNatural products which grow in adverse conditions, such as fungi, are a possible rich source of biologically active compounds with potential for drug discovery [1,2]. We have recently reported the growth inhibitory activity of extracts from Clitocybe alexandri, a wild mushroom collected from the northwest of Portugal, in human tumour cell lines (NCI-H460, MCF-7, HCT-15 and AGS) [3]. The ethanolic extract was the most potent one, particularly in NCI-H460 cells (GI50 24.8 ± 2.3 μg/ml) [3]. The aim of this work was to further study the potential of this extract as a possible source of cytotoxic compounds. In order to understand the mechanism of action of this extract in the NCI-H460 cells, the effects on cell cycle (following PI labelling) and apoptosis (following AnnexinV/PI labelling) were evaluated by flow cytometry. Furthermore, the involvement of proteins related to cellular apoptosis was investigated by Western blot. The ethanolic extract was characterized regarding its phenolic composition by HPLC-DAD/ESI. The isolated compounds were also studied regarding their growth inhibitory activity, by SBR assay. The effect of isolated or combined compounds on viable cell number was also evaluated using the Trypan blue exclusion assay. It was observed that the Clitocybe alexandri extract induced an S-phase cell cycle arrest and increased the percentage of apoptotic cells. In addition, treatment with the GI50 concentration for 48h caused an increase in the levels of wt p53, cleaved caspase-3 and cleaved PARP. The main components identified in this extract were protocatechuic acid (16.4 ± 2.2 mg/kg dw), p-hydroxybenzoic acid (8.3 ± 0.4 mg/kg) and cinnamic acid (6.4 ± 0.3 mg/kg). Cinnamic acid was found to be the most potent compound regarding cell growth inhibition. Finally, it was verified that their concomitant use provided the strongest decrease in viable cell number.por
dc.identifier.citationVaz, Josiana A.; Almeida, Gabriela M.; Ferreira, Diana; Ferreira, Isabel C.F.R.; Martins, Anabela; Vasconcelos, M. Helena (2011). Clitocybe alexandri extract induces apoptosis in a lung cancer cell line: identification of phenolic acids with cytotoxic potential. In XX Porto Cancer Meeting. Drug resistance in cancer: from biology to molecular targets and drugs. Portopor
dc.identifier.urihttp://hdl.handle.net/10198/6036
dc.language.isoengpor
dc.peerreviewedyespor
dc.relationPortuguese Wild Mushrooms: Chemical characterization and functional study of antiproliferative and proapoptotic properties in cancer cell lines
dc.relationPOTENCIAL NUTRACEUTICO DE COGUMELOS SILVESTRES DO NORDESTE DE PORTUGAL: ESTUDO DE PROPRIEDADES ANTIO-XIDANTES, ANTIPROLIFERATIVAS E INDUTORAS DA APOPTOSE
dc.titleClitocybe alexandri extract induces apoptosis in a lung cancer cell line: identification of phenolic acids with cytotoxic potentialpor
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitlePortuguese Wild Mushrooms: Chemical characterization and functional study of antiproliferative and proapoptotic properties in cancer cell lines
oaire.awardTitlePOTENCIAL NUTRACEUTICO DE COGUMELOS SILVESTRES DO NORDESTE DE PORTUGAL: ESTUDO DE PROPRIEDADES ANTIO-XIDANTES, ANTIPROLIFERATIVAS E INDUTORAS DA APOPTOSE
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FAGR-ALI%2F110062%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F43653%2F2008/PT
oaire.citation.conferencePlacePortopor
oaire.citation.titleXX Porto Cancer Meeting. Drug resistance in cancer: from biology to molecular targets and drugs, 28 e 29 de Abril de 2011por
oaire.fundingStream5876-PPCDTI
person.familyNameVaz
person.familyNameFerreira
person.familyNameMartins
person.givenNameJosiana A.
person.givenNameIsabel C.F.R.
person.givenNameAnabela
person.identifier144781
person.identifier.ciencia-id171B-88BA-64F5
person.identifier.ciencia-id9418-CF95-9919
person.identifier.ciencia-id7B18-A810-6B93
person.identifier.orcid0000-0002-6989-1169
person.identifier.orcid0000-0003-4910-4882
person.identifier.orcid0000-0001-6218-4413
person.identifier.ridE-8500-2013
person.identifier.ridG-5488-2013
person.identifier.scopus-author-id16305323200
person.identifier.scopus-author-id36868826600
person.identifier.scopus-author-id7203013518
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typeconferenceObjectpor
relation.isAuthorOfPublication1c1a2cb9-5a36-47ba-bca0-ffcbef28df18
relation.isAuthorOfPublicationbd0d1537-2e03-41fb-b27a-140af9c35db8
relation.isAuthorOfPublication383370dd-10e5-40e6-9a15-f2201fe95581
relation.isAuthorOfPublication.latestForDiscovery1c1a2cb9-5a36-47ba-bca0-ffcbef28df18
relation.isProjectOfPublication59db4b60-8cc2-4f07-a25a-8cfe78f2778f
relation.isProjectOfPublicationedecd8db-6ac9-4523-bb6e-ac66fae6ad7e
relation.isProjectOfPublication.latestForDiscoveryedecd8db-6ac9-4523-bb6e-ac66fae6ad7e

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Nacional 55.pdf
Size:
451.43 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CIMO - Resumos em Proceedings Não Indexados à WoS/Scopus