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Banana Peels as Substrate for Lactic Acid Production: Upstream Fermentation Bioprocess

2026Artigo científico Acesso aberto
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datacite.subject.fosCiências Agrárias::Biotecnologia Agrária e Alimentar
datacite.subject.fosEngenharia e Tecnologia::Engenharia Química
datacite.subject.fosEngenharia e Tecnologia::Biotecnologia Ambiental
datacite.subject.sdg09:Indústria, Inovação e Infraestruturas
datacite.subject.sdg04:Educação de Qualidade
datacite.subject.sdg12:Produção e Consumo Sustentáveis
dc.contributor.authorPedrosa, Mariana C.
dc.contributor.authorHeleno, Sandrina A.
dc.contributor.authorAlvarez, Cristina
dc.contributor.authorSusmozas, Ana
dc.contributor.authorBallesteros, Ignacio
dc.contributor.authorPintado, Manuela
dc.contributor.authorBarros, Lillian
dc.contributor.authorCarocho, Márcio
dc.date.accessioned2026-06-30T15:32:54Z
dc.date.available2026-06-30T15:32:54Z
dc.date.issued2026
dc.description.abstractBanana peels account for around 30–40% of the banana’s weight and represent an industrial waste. This biomass can be used as an eco-friendly solution for biotechnological bioprocesses. Still, due to the fibrous nature of banana peels, pretreatment becomes essential to enhance the effectiveness of enzymes on the hydrolysis of complex carbohydrates. This study aims to determine the best pretreatment and enzymatic hydrolysis conditions to produce a fermentation substrate suitable for producing lactic acid from the peels. Methods: A diluted H2SO4 (0.5% and 0.25%) was used in an autoclave for 10 min at 121 °C. The pretreated material was subjected to several enzymatic hydrolysis tests in which two variables were evaluated for their influence: solid load (10, 15 and 20%), and H2SO4 concentration (0.5 and 0.25% v/v). Commercial enzymes (cellulolytic enzyme mixtures and pectinase) were used. Results: All enzymatic hydrolysis yields of cellulose referring to glucose released in the hydrolysates surpassed 60% in all the conditions tested after pretreatment. After evaluating the pretreatment efficiency, the ideal parameters were defined as 0.25% H2SO4, and cellulolytic enzyme mixtures with 15 and 20% solid load. Subsequently, a screening was carried out in MRS + broth with the most suitable pH and temperature conditions for lactic acid production by Lacticaseibacillus rhamnosus. Conclusions: The results showed the potential of BP medium for lactic acid production, with yields of 0.91 g/g sugar in sugar-containing media from enzymatic hydrolysis at 15% solid load and 0.82 g/g sugar in media from 20% solid load assays.eng
dc.description.sponsorshipThis work was supported by national funds through FCT/MCTES (PIDDAC): CIMO, UIDB/00690/2020 (DOI: https://doi.org/10.54499/UIDB/00690/2020) and UIDP/00690/2020 (DOI: https://doi.org/10.54499/UIDP/00690/2020); and SusTEC, LA/P/0007/2020 (DOI: https://doi.org/10.54499/LA/P/0007/2020). RESOPLA (Grant TED2021-132024B-C22 funded by MCIU/AEI/ https://doi.org/10.13039/501100011033 and by the “European Union NextGenerationEU/PRTR). To National funding by FCT- Foundation for Science and Technology, through the institutional scientific employment programcontract with S.A. Heleno and L. Barros (CEEC-INST, DOI: https://doi.org/10.54499/CEECINST/00107/2021/CP2793/CT0002), as well as M. Carocho for his individual contract (CEEC-IND/00831/2018), while M.C. Pedrosa’s acknowledges FCT for her PhD grant (DOI https://doi.org/10.54499/2021.04531.BD).
dc.identifier.citationPedrosa, Mariana C.; Heleno, Sandrina A.; Alvarez, Cristina; Susmozas, Ana; Ballesteros, Ignacio; Pintado, Manuela; Barros, Lillian; Carocho, Márcio (2026). Banana Peels as Substrate for Lactic Acid Production: Upstream Fermentation Bioprocess. Waste and Biomass Valorization. ISSN 1877-2641. 17:6, p. 3269-3285
dc.identifier.doi10.1007/s12649-025-03323-y
dc.identifier.issn1877-2641
dc.identifier.urihttp://hdl.handle.net/10198/36960
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer
dc.relationMountain Research Center - UIDB/00690/2020
dc.relationAssociate Laboratory for Sustainability and Tecnology in Mountain Regions - LA/P/0007/2020
dc.relationPlantPack: Volatile plant compounds as active packaging agents: prospection, extraction optimization, in silico studies and innovative applications
dc.relationMountain Research Center - UIDP/00690/2020
dc.relationInvestigação em alimentos e soluções de base biológica - CEECINST/00107/2021/CP2793/CT0002
dc.relationSUSPLA: Development of PLA Biofilms from Food Waste, Incorporated with Bioactive Preserving Molecules
dc.relation.ispartofWaste and Biomass Valorization
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBiowaste
dc.subjectCarbon source
dc.subjectLactic acid
dc.subjectHydrolysis
dc.titleBanana Peels as Substrate for Lactic Acid Production: Upstream Fermentation Bioprocesseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberUIDB/00690/2020
oaire.awardNumberLA/P/0007/2020
oaire.awardNumberCEECIND/00831/2018/CP1578/CT0001
oaire.awardNumberUIDP/00690/2020
oaire.awardNumberCEECINST/00107/2021/CP2793/CT0002
oaire.awardNumber2021.04531.BD
oaire.awardTitleMountain Research Center - UIDB/00690/2020
oaire.awardTitleAssociate Laboratory for Sustainability and Tecnology in Mountain Regions - LA/P/0007/2020
oaire.awardTitlePlantPack: Volatile plant compounds as active packaging agents: prospection, extraction optimization, in silico studies and innovative applications
oaire.awardTitleMountain Research Center - UIDP/00690/2020
oaire.awardTitleInvestigação em alimentos e soluções de base biológica - CEECINST/00107/2021/CP2793/CT0002
oaire.awardTitleSUSPLA: Development of PLA Biofilms from Food Waste, Incorporated with Bioactive Preserving Molecules
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0007%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2018/CEECIND%2F00831%2F2018%2FCP1578%2FCT0001/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT
oaire.awardURIhttps://doi.org/10.54499/CEECINST/00107/2021/CP2793/CT0002
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//2021.04531.BD/PT
oaire.citation.endPage3285
oaire.citation.issue6
oaire.citation.startPage3269
oaire.citation.titleWaste and Biomass Valorization
oaire.citation.volume17
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamCEEC IND 2018
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNamePedrosa
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person.familyNameBarros
person.familyNameCarocho
person.givenNameMariana C.
person.givenNameSandrina A.
person.givenNameLillian
person.givenNameMárcio
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person.identifier.orcid0000-0002-9050-5189
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