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First study on the root endophytic fungus Trichoderma hamatum as an entomopathogen: development of a fungal bioinsecticide against cotton leafworm (Spodoptera littoralis)

2023Artigo científico Acesso aberto
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dc.contributor.authorLana, Maite
dc.contributor.authorSimo, Oihane
dc.contributor.authorVelasco, Pablo
dc.contributor.authorRodriguez, Victor M.
dc.contributor.authorCaballero, Primitivo
dc.contributor.authorPoveda, Jorge
dc.date.accessioned2020-04-27T09:41:30Z
dc.date.available2020-04-27T09:41:30Z
dc.date.issued2023
dc.description.abstractCotton leaf worm (Spodoptera littoralis) is a pest that produces important losses in horticultural and ornamental crops in greenhouse, being classified as quarantine pest A2 by EPPO. One of the strategies proposed to control agricultural pests in a health and environmentally friendly way is biological control with entomopathogenic fungi. The genus of filamentous fungi Trichoderma includes different species with direct (infection, antibiosis, anti-feeding, etc.) and indirect (systemic activation of plant defenses) insecticidal capacity, however, the species T. hamatum has never been described previously as entomopathogenic. In this work, the entomopathogenic capacity of T. hamatum on S. littoralis L3 larvae was analyzed by applying spores and fungal filtrates (topically and orally). Infection by spores was compared with the commercial entomopathogenic fungus Beauveria bassiana, obtaining similar results with respect to the production of larval mortality. Oral application of spores reported high mortality and fungal colonization of larvae, however, T. hamatum did not show chitinase activity when grown in the presence of S. littoralis tissues. Therefore, infection of S. littoralis larvae by T. hamatum is through natural openings such as mouth, anus or spiracles. With respect to the application of filtrates, only those obtained from the liquid culture of T. hamatum in contact with S. littoralis tissues reported a significant reduction in larval growth. Metabolomic analysis of the filtrates determined that the filtrate with insecticidal capacity presented the siderophore rhizoferrin in large quantities, which could be responsible for this activity. However, the production of this siderophore had never been previously described in Trichoderma and its insecticidal capacity was unknown. In conclusion, T. hamatum presents entomopathogenic capacity against S. littoralis larvae through the application of spores and filtrates, and both ways could be the basis for the development of efficient bioinsecticides against the pest.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLana, Maite; Simo, Oihane; Velasco, Pablo; Rodriguez, Victor M.; Caballero, Primitivo; Poveda, Jorge (2023). First study on the root endophytic fungus Trichoderma hamatum as an entomopathogen: development of a fungal bioinsecticide against cotton leafworm (Spodoptera littoralis). Microbiological Research. eISSN 1618-0623. 270, p. 1-9pt_PT
dc.identifier.doi10.1016/j.micres.2023.127334
dc.identifier.eissn1618-0623
dc.identifier.issn0944-5013
dc.identifier.urihttp://hdl.handle.net/10198/21824
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectTrichodermapt_PT
dc.subjectBeauveria bassianapt_PT
dc.subjectFungi entomopathogenpt_PT
dc.subjectChitinasept_PT
dc.subjectRhizoferrinpt_PT
dc.titleFirst study on the root endophytic fungus Trichoderma hamatum as an entomopathogen: development of a fungal bioinsecticide against cotton leafworm (Spodoptera littoralis)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleMicrobiological Researchpt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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