Publication
Polymorphisms in cytochrome P450 versus cline distribution of evolutionary lineages in Apis mellifera iberiensis
| dc.contributor.author | Muñoz, Irene | |
| dc.contributor.author | Henriques, Dora | |
| dc.contributor.author | De la Rúa, Pilar | |
| dc.contributor.author | Pinto, M. Alice | |
| dc.date.accessioned | 2019-02-26T10:54:32Z | |
| dc.date.available | 2019-02-26T10:54:32Z | |
| dc.date.issued | 2018 | |
| dc.description.abstract | Honey bees (Apis mellifera) are the most prominent and economically important pollinator species worldwide. However, the reported decline of its populations in several regions of the world over the last decades is of concern. The causes are manifold, including the spread of pathogens and parasites, malnutrition and habitat loss, climate change and xenobiotics, especially pesticides. Among the main mechanisms used by insects to cope with the adverse effects of xenobiotics is the metabolic resistance mediated mainly by three superfamilies of enzymes: the cytochrome P450 monooxygenases, the glutathione transferases and the carboxylesterases. We hypothesize that the genetic background influences the sensitivity to pesticides or detoxification capacity of different honey bee populations, ecotypes and subspecies. The Iberian Peninsula provides an interesting scenario to study the genetic variability of the cytochrome P450 genes given the co-occurrence of two clinally distributed evolutionary lineages, as a result of secondary contact. In this study, the genetic variability of six genes of the cytochrome P450 superfamily (CYP6AS3, CYP6AS4, CYP6AS5, CYP6AS7, CYP6AS12 and CYP6AS17) was analyzed in the Iberian honey bee (Apis mellifera iberiensis) to provide more information on the mechanisms of resistance to xenobiotics and to identify the genetic variation involved in local adaptation. Genomic signal of selective sweeps was detected in three genes, of which CYP6AS5 presents the highest number of point mutations under selection, being proposed as a candidate gene to perform gene expression studies. We discuss the correlation between the variability of P450 genes and the distribution of the evolutionary lineages in the Iberian Peninsula. The identification of polymorphisms in these genes promises to shed light on the relationship between diversity and xenobiotic tolerance of A. m. iberiensis. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Muñoz, Irene; Henriques, Dora; De la Rúa, P.; Pinto, M. Alice (2018). Polymorphisms in cytochrome P450 versus cline distribution of evolutionary lineages in Apis mellifera iberiensis. In EURBEE 2018: 8th European Conference of Apidology. Ghent, Belgium | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10198/18974 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | Apis mellifera iberiensis | pt_PT |
| dc.subject | Cytochrome P450 | pt_PT |
| dc.subject | Xenobiotic tolerance | pt_PT |
| dc.subject | Genetic variability | pt_PT |
| dc.title | Polymorphisms in cytochrome P450 versus cline distribution of evolutionary lineages in Apis mellifera iberiensis | pt_PT |
| dc.type | conference object | |
| dspace.entity.type | Publication | |
| oaire.citation.conferencePlace | Ghent, Belgium | pt_PT |
| oaire.citation.title | EURBEE 2018: 8th European Conference of Apidology | pt_PT |
| person.familyName | Henriques | |
| person.familyName | Pinto | |
| person.givenName | Dora | |
| person.givenName | Maria Alice | |
| person.identifier.ciencia-id | 291F-986F-07DA | |
| person.identifier.ciencia-id | F814-A1D0-8318 | |
| person.identifier.orcid | 0000-0001-7530-682X | |
| person.identifier.orcid | 0000-0001-9663-8399 | |
| person.identifier.scopus-author-id | 55761737300 | |
| person.identifier.scopus-author-id | 8085507800 | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | conferenceObject | pt_PT |
| relation.isAuthorOfPublication | d2abd09f-a90c-4cfb-9a60-7fc32f56184d | |
| relation.isAuthorOfPublication | 0667fe04-7078-483d-9198-56d167b19bc5 | |
| relation.isAuthorOfPublication.latestForDiscovery | d2abd09f-a90c-4cfb-9a60-7fc32f56184d |