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Chemical characterization and antimicrobial activity of ozonized oil against methicillin resistant Staphylococcus aureus

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dc.contributor.authorSilva, Vanessa
dc.contributor.authorPeirone, Cecília
dc.contributor.authorAmaral, Joana S.
dc.contributor.authorMarques-Magallanes, José A.
dc.contributor.authorIgrejas, Getúlio
dc.contributor.authorPoeta, Patrícia
dc.date.accessioned2020-07-14T08:47:16Z
dc.date.available2020-07-14T08:47:16Z
dc.date.issued2018
dc.description.abstractMethicillin resistant Staphylococcus aureus (MRSA) are frequently found in infected skin wounds due to the exposure of deep tissues to the bacteria, in particular in infected diabetic foot ulcers in which the prevalence can be between 15 to 40% [1]. Clinical MRSA are usually resistant to most classes of antibiotics and, therefore, it is necessary to investigate new antimicrobial agents. Ozone has a recognized bactericidal activity and has been widely used as a clinical therapeutic agent for chronic wounds, such as, ulcers and other diabetic lesions, due to its wound healing ability [2]. This study aimed to characterize ozone oil suspensions and their activity against MRSA isolated from infected diabetic foot ulcers. Six ozonized oils with different ozonization doses were tested by disk diffusion method. The oils were prepared from a 50:50 mixture of extra virgin olive oil and refined sunflower oil and submitted to a gas stream of O2/O3 mixture at a concentration of 75 μg/ml of ozone in continuous flow (4 L/min). The oil content in fatty acid methyl esters after ozonization were investigated using a Bruker® SCION 436-GC gas chromatograph equipped with a flame ionization detector (GC-FID). All ozone suspension oils tested showed antimicrobial activity against all strains. The ozone suspension oil with the highest ozonization dose originated clear zones with a maximum of 12 mm. Composition of fatty acids in ozonized oils showed gradual decrease in unsaturated fatty acids with gradual increase in ozone doses. Overall, there was a decrease in the total fatty acid investigated and an increase in the content of unknown oxidized fatty acid with the increase of ozonization period. Our results indicate that ozonized oils have a significant antimicrobial activity, however, it is still unclear which compounds may be responsible for the bactericidal action.pt_PT
dc.description.sponsorshipVanessa Silva is grateful to FCT (Fundação para a Ciência e Tecnologia) for her PhD grant SFRH/BD/137947/2018pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSilva, Vanessa ; Peirone, Cecília; Amaral, J.S.; Marques-Magallanes, José A.; Igrejas, Getúlio; Poeta, Patrícia (2018). Chemical characterization and antimicrobial activity of ozonized oil against methicillin resistant Staphylococcus aureus. In 3rd International Caparica Christmas Conference on Sample Treatment. Costa da Caparicapt_PT
dc.identifier.urihttp://hdl.handle.net/10198/22470
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectStaphylococcus aureuspt_PT
dc.titleChemical characterization and antimicrobial activity of ozonized oil against methicillin resistant Staphylococcus aureuspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceCosta da Caparica, Portugalpt_PT
oaire.citation.endPage142pt_PT
oaire.citation.startPage142pt_PT
oaire.citation.titleProceedings Book 3rd International Caparica Christmas Conference on Sample Treatmentpt_PT
person.familyNameAmaral
person.familyNameIgrejas
person.givenNameJoana S.
person.givenNameGetúlio
person.identifier.ciencia-id5319-7DE8-BEDA
person.identifier.orcid0000-0002-3648-7303
person.identifier.orcid0000-0002-6820-8858
person.identifier.ridM-8571-2013
person.identifier.scopus-author-id47761255900
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication42be2cf4-adc4-4e7f-ac60-7aab515b38cd
relation.isAuthorOfPublicationab4092ec-d1b1-4fe0-b65a-efba1310fd5a
relation.isAuthorOfPublication.latestForDiscoveryab4092ec-d1b1-4fe0-b65a-efba1310fd5a

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