Repository logo
 
Publication

Leccinum vulpinum antitumor potential: which cell biological functions may be affected?

2016Conference object Open access
Simple item page
dc.contributor.authorReis, Filipa S.
dc.contributor.authorSousa, Diana
dc.contributor.authorMartins, Anabela
dc.contributor.authorMorales, Patricia
dc.contributor.authorFerreira, Isabel C.F.R.
dc.date.accessioned2017-01-17T11:36:34Z
dc.date.available2017-01-17T11:36:34Z
dc.date.issued2016
dc.description.abstractBesides being an excellent choice as food for their high nutritional value, mushrooms have been identified as products with bioactive properties, including antitumor potential. Herein, a phenolic extract, obtained from the edible mushroom Leccinum vulpinum Watling, which was rich essentially in hydroxybenzoic acids (i.e., gallic acid, protocatechuic acid and p-hydroxybenzoic acid) was analyzed. The extract was tested against a panel of four different human tumor cell lines (MCF-7, NCI-H460, HCT-15 and AGS), to perform an initial screening of its impact on the growth of these cells. Since the proliferation of all the tumor cell lines was inhibited, and given the evidence of an inverse relationship between mushroom’s consumption and breast cancer risk reduction [1], a detailed study was performed on breast adenocarcinoma cells. Some functional assays were carried out, namely cell proliferation, cell cycle profile and apoptosis. The potential of the extract as an inducer of DNA damage was also evaluated. Overall, the extract decreased the cellular proliferation (cells treated with the GI75 concentration of the extract reduced significantly the percentage of cells in the S-phase) and induced apoptosis (cells treated with the GI50 concentration of the extract increased the percentage of cells undergoing apoptosis to 13.4% and cells treated with the GI75 concentration increased that percentage to 27%). The obtained results suggest that the extract also causes cellular DNA damage, since some proteins involved in the repair of DNA damage (PARP), are increased. Through the Comet assay, a significant increase of the percentage of Tail DNA upon treatment with 250 mg/mL of the extract, was also observed.pt_PT
dc.description.sponsorshipIPATIMUP integrates the i3S Research Unit, which is partially supported by FCT, the Portuguese Foundation for Science and Technology (FCT). The authors are grateful to FCT for the grant of F.S. Reis (SFRH/BD/111753/2015), D. Sousa (SFRH/BD/98054/2013), and FCT and FEDER for CIMO (UID/AGR/00690/2013) support.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationReis, Filipa S.; Sousa, Diana; Martins, Anabela; Morales, Patricia; Ferreira, Isabel C.F.R. (2016). Leccinum vulpinum antitumor potential: which cell biological functions may be affected? In XIII Encontro de Química dos Alimentos. Porto. ISBN 978-989-8124-14-2pt_PT
dc.identifier.isbn978-989-8124-14-2
dc.identifier.urihttp://hdl.handle.net/10198/13832
dc.language.isoporpt_PT
dc.peerreviewedyespt_PT
dc.relationStudy of nutritional, antioxidant, anti-proliferative and programmed cell death induction properties of wild mushrooms
dc.relationUnderstanding the interactions between P-glycoprotein and microRNAs in the transfer of drug resistance by extracellular vesicles
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleLeccinum vulpinum antitumor potential: which cell biological functions may be affected?pt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleStudy of nutritional, antioxidant, anti-proliferative and programmed cell death induction properties of wild mushrooms
oaire.awardTitleUnderstanding the interactions between P-glycoprotein and microRNAs in the transfer of drug resistance by extracellular vesicles
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F111753%2F2015/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F98054%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAGR%2F00690%2F2013/PT
oaire.citation.conferencePlacePortopt_PT
oaire.citation.title13º Encontro de Química dos Alimentospt_PT
oaire.fundingStreamFARH
oaire.fundingStream5876
person.familyNameReis
person.familyNameMartins
person.familyNameFerreira
person.givenNameFilipa S.
person.givenNameAnabela
person.givenNameIsabel C.F.R.
person.identifier144781
person.identifier.ciencia-id391F-AFE1-64C7
person.identifier.ciencia-id7B18-A810-6B93
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0002-9159-0530
person.identifier.orcid0000-0001-6218-4413
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridI-2093-2013
person.identifier.ridG-5488-2013
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id36982144400
person.identifier.scopus-author-id7203013518
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicationb8d384ae-2134-4735-93a6-0d2febbf9220
relation.isAuthorOfPublication383370dd-10e5-40e6-9a15-f2201fe95581
relation.isAuthorOfPublicationbd0d1537-2e03-41fb-b27a-140af9c35db8
relation.isAuthorOfPublication.latestForDiscoverybd0d1537-2e03-41fb-b27a-140af9c35db8
relation.isProjectOfPublicationb9a0719b-2b89-41c3-9f3a-1f116deacb83
relation.isProjectOfPublication61d09d75-f9a5-4923-89f2-cfd8dbf89bc0
relation.isProjectOfPublicationcfb6e380-87c4-4772-8c2c-76799a53d6df
relation.isProjectOfPublication.latestForDiscoveryb9a0719b-2b89-41c3-9f3a-1f116deacb83

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Poster Nac. 253.pdf
Size:
337.37 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CIMO - Resumos em Proceedings Não Indexados à WoS/Scopus