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High hydrostatic pressure as a tool to improve the antioxidant activity of watercress extracts

2021Conference object Open access
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dc.contributor.authorPinela, José
dc.contributor.authorAñibarro-Ortega, Mikel
dc.contributor.authorBarros, Lillian
dc.contributor.authorSaraiva, Jorge A.
dc.contributor.authorFerreira, Isabel C.F.R.
dc.date.accessioned2022-01-21T15:46:39Z
dc.date.available2022-01-21T15:46:39Z
dc.date.issued2021
dc.description.abstractWatercress (Nasturtium officinale R. Br.) belongs to the Brassicaceae family together with broccoli, cabbage, mustard and Brussels sprouts. This semiaquatic plant is source of bioactive phytochemicals such as polyphenols, glucosinolates, and carotenoids, which have received great importance due to their several health benefits linked to antioxidant activity [1,2]. To obtain plant extracts, the extraction method must be thought out carefully to ensure successful recovery of the bioactive compounds. Therefore, the selection of an appropriate extraction method is important for the production of high quality antioxidant extracts, otherwise the extraction can lead to degradation of the target compounds. High pressure processing (HPP) has been used in the extraction of bioactive molecules from plant matrices with very promising results because, since no heat is used, it avoids harmful effects on thermosensitive compounds and, consequently, preserves better their biological activities. HPP provides higher extraction yields and selectivity for target compounds than conventional extraction methods [3]. Pressure can disrupt plant tissues and enhance mass transfer of solvent into the materials and the soluble constituents into the solvent. Thus, this study was carried out to outline the best extraction conditions to obtain antioxidant extracts from watercress. Powdered watercress was processed according to a central composite design of 20 runs, in which five levels of time (1.5–33.5 min), pressure (0.1–600 MPa) and ethanol concentration (0–100%, v/v) were combined. The antioxidant activity of the obtained extracts was measured in vitro by the DPPH• scavenging activity and reducing power assays, and the results were presented as EC50 values and used as response variables in the optimization by response surface methodology. Design Expert software was used for regression and graphical analysis of the data. A quartic polynomial equation that correlates the response as a function of the independent variables and their interaction was developed. The models were successfully fitted to the experimental data, statistically validated based on high F-values (>64.9) and R2adj (>0.96), and used to navigate the design space and predict the optimal extraction conditions. Considering both response criteria, the HHP conditions that sustainably maximized the extract antioxidant activity required a low processing time (1.5 min) and an intermediate pressure (319 MPa) and solvent concentration (55% ethanol, v/v), and yielded EC50 values of 0.45 mg/mL and 0,41 mg/mL for the DPPH and reducing power assays, respectively. HHP extraction revealed so to be a time-saving process comparatively with conventional methods.pt_PT
dc.description.sponsorshipTo FCT and FEDER under Programme PT2020 for financial support to CIMO (UIDB/00690/2020); to FCT/MEC for the financial support to QOPNA (FCT UID/QUI/00062/2019) and LAQV-REQUIMTE (UIDB/50006/2020), through national funds and, where applicable, co-financed by the FEDER, within the PT2020 Partnership Agreement; to the contracts of J.Pinela and L.Barros (national funding by FCT, through the institutional scientific employment program- contract); and to the grant of M. Añibarro-Ortega (project ValorNatural, Norte-01-0247-FEDER-024479). Work funded by FEDER through the Regional Operational Program North 2020, within the scope of Projects NORTE-01- 0145-FEDER-023289: DeCodE and Norte-01-0247-FEDER-024479: ValorNatural®.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPinela, José; Añibarro-Ortega, Mikel; Barros, Lillian; Saraiva, Jorge A.; Ferreira, Isabel C.F.R. (2021). Highhydrostatic pressure as a tool to improve the antioxidant activity of watercress extracts. In 7th Portuguese Young Chemists Meeting. ISBN 978-989-8124-31-0pt_PT
dc.identifier.isbn978-989-8124-31-0
dc.identifier.urihttp://hdl.handle.net/10198/24848
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationMountain Research Center
dc.relationOrganic Chemistry, Natural Products and Food Stuffs
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectNasturtium officinale R. Br.pt_PT
dc.subjectBrassicaceae familypt_PT
dc.titleHigh hydrostatic pressure as a tool to improve the antioxidant activity of watercress extractspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardTitleOrganic Chemistry, Natural Products and Food Stuffs
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FQUI%2F00062%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT
oaire.citation.conferencePlaceBragança, Portugalpt_PT
oaire.citation.title7th Portuguese Young Chemists Meetingpt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNamePinela
person.familyNameAñibarro-Ortega
person.familyNameBarros
person.familyNameFerreira
person.givenNameJosé
person.givenNameMikel
person.givenNameLillian
person.givenNameIsabel C.F.R.
person.identifier469085
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person.identifier.orcid0000-0001-7523-1637
person.identifier.orcid0000-0002-2835-5079
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridB-4466-2014
person.identifier.ridJ-3600-2013
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id54392272800
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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